Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2143716..2144230 Replicon chromosome
Accession NZ_CP096526
Organism Staphylococcus aureus strain RIVM_M087195

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag M1F51_RS10495 Protein ID WP_000581792.1
Coordinates 2143716..2143925 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag M1F51_RS10500 Protein ID WP_000138932.1
Coordinates 2143937..2144230 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F51_RS10475 (M1F51_10450) 2139227..2140582 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
M1F51_RS10480 (M1F51_10455) 2140597..2141667 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
M1F51_RS10485 (M1F51_10460) 2141985..2143187 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
M1F51_RS10490 (M1F51_10465) 2143451..2143588 - 138 WP_000828354.1 Lmo0850 family protein -
M1F51_RS10495 (M1F51_10470) 2143716..2143925 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
M1F51_RS10500 (M1F51_10475) 2143937..2144230 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
M1F51_RS10505 (M1F51_10480) 2144396..2145880 + 1485 WP_267355475.1 cardiolipin synthase -
M1F51_RS10510 (M1F51_10485) 2145908..2146555 + 648 WP_267355477.1 HD domain-containing protein -
M1F51_RS10515 (M1F51_10490) 2147112..2147174 + 63 Protein_2030 hypothetical protein -
M1F51_RS10520 (M1F51_10495) 2147152..2148467 - 1316 Protein_2031 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T243086 WP_000581792.1 NZ_CP096526:c2143925-2143716 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T243086 NZ_OW969711:3397262-3397480 [Citrobacter koseri]
ATGTCCGATAAACCATTAACGAAAACAGATTATTTGATGCGTTTACGACGCTGCCAGACAATAGACACGCTGGAACGTGT
AATAGAGAAAAATAAATATGAATTGTCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGATCACCGTCTTGCAGAAT
TGACGATGAATAAGCTCTACGACAAGATTCCCACCTCCGTGTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT243086 WP_000138932.1 NZ_CP096526:c2144230-2143937 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT243086 NZ_OW969711:3396860-3397234 [Citrobacter koseri]
ATGGATGAGTACTCACCCAAAAGACATGATATCGCACAGCTTAAATTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCACGGTTGGGTTAATGACCCTACTTCGGCAATCAATCTTCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGCACAGATAGATGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAATGCGCAGGATCTGCAAAAATGGCGAAAATCAGGAAACCGCCT
GTTTCGCTGCTTCGTCAATGCCACAAGGGCGAATCCTGTTAGCTTGTCATGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References