Detailed information of TA system TA243085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2123979..2124508
• Replicon: chromosome
• Accession: NZ_CP096526
• Organism: Staphylococcus aureus strain RIVM_M087195

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M1F51_RS10405
• Protein ID: WP_000621175.1
• Coordinates: 2123979..2124341 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: M1F51_RS10410
• Protein ID: WP_000948331.1
• Coordinates: 2124338..2124508 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F51_RS10385 (M1F51_10360)	2120957..2121727	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
M1F51_RS10390 (M1F51_10365)	2121702..2122181	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
M1F51_RS10395 (M1F51_10370)	2122183..2122509	-	327	WP_001052491.1	anti-sigma factor antagonist	-
M1F51_RS10400 (M1F51_10375)	2122628..2123629	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
M1F51_RS10405 (M1F51_10380)	2123979..2124341	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1F51_RS10410 (M1F51_10385)	2124338..2124508	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M1F51_RS10415 (M1F51_10390)	2124593..2125741	-	1149	WP_001281139.1	alanine racemase	-
M1F51_RS10420 (M1F51_10395)	2125807..2126166	-	360	WP_000581197.1	holo-ACP synthase	-
M1F51_RS10425 (M1F51_10400)	2126170..2126661	-	492	WP_001286980.1	PH domain-containing protein	-
M1F51_RS10430 (M1F51_10405)	2126654..2128231	-	1578	WP_001294650.1	PH domain-containing protein	-
M1F51_RS10435 (M1F51_10410)	2128224..2128703	-	480	WP_001287081.1	hypothetical protein	-
M1F51_RS10440 (M1F51_10415)	2128912..2129472	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T243085 WP_000621175.1 NZ_CP096526:c2124341-2123979 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T243085 NZ_OW969711:c2942584-2942099 [Citrobacter koseri]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TAACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGACCCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATAGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAGTCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT243085 WP_000948331.1 NZ_CP096526:c2124508-2124338 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT243085 NZ_OW969711:c2942838-2942572 [Citrobacter koseri]
ATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGATGCAGCTGCGGAAATCCTTCA
CAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCCTTGACCGCCGTGTATTTAACT
TTAACGATGAGCAATATACAGAGTTCATCGACATGCTTGATGCGCCGGTCGCAGATGATCCCGTTATCGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1