Detailed information of TA system TA243077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2111545..2112074
• Replicon: chromosome
• Accession: NZ_CP096522
• Organism: Staphylococcus aureus strain RIVM_M085090

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M1F52_RS10320
• Protein ID: WP_000621175.1
• Coordinates: 2111545..2111907 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: M1F52_RS10325
• Protein ID: WP_000948331.1
• Coordinates: 2111904..2112074 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1F52_RS10300 (M1F52_10280)	2108523..2109293	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
M1F52_RS10305 (M1F52_10285)	2109268..2109747	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
M1F52_RS10310 (M1F52_10290)	2109749..2110075	-	327	WP_001052491.1	anti-sigma factor antagonist	-
M1F52_RS10315 (M1F52_10295)	2110194..2111195	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
M1F52_RS10320 (M1F52_10300)	2111545..2111907	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1F52_RS10325 (M1F52_10305)	2111904..2112074	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M1F52_RS10330 (M1F52_10310)	2112159..2113307	-	1149	WP_001281139.1	alanine racemase	-
M1F52_RS10335 (M1F52_10315)	2113373..2113732	-	360	WP_000581197.1	holo-ACP synthase	-
M1F52_RS10340 (M1F52_10320)	2113736..2114227	-	492	WP_001286980.1	PH domain-containing protein	-
M1F52_RS10345 (M1F52_10325)	2114220..2115797	-	1578	WP_001294650.1	PH domain-containing protein	-
M1F52_RS10350 (M1F52_10330)	2115790..2116269	-	480	WP_001287081.1	hypothetical protein	-
M1F52_RS10355 (M1F52_10335)	2116478..2117038	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T243077 WP_000621175.1 NZ_CP096522:c2111907-2111545 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T243077 NZ_OW969691:2287045-2287221 [Citrobacter koseri]
GTGAAGCAGAGCGAATTCAGACGCTGGCTTGAATCTCAGGGAGTCGATGTAACAAATGGAACGAATCATTTGAAGTTGAG
ATATCACGACAAACGAAGCGTCATGCCCAGACATCCTGGTGATGAAATTAAAGACACCCTGCGTAAAGCAATACTAAAGC
AGCCTGGTTTGAATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT243077 WP_000948331.1 NZ_CP096522:c2112074-2111904 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT243077 NZ_OW969691:2287268-2287684 [Citrobacter koseri]
ATGCGTTATCCCGTCAATCTCATTCCCGCAGAAGAAGGGGGTTATGTTGTGTCGTTCCCGGACATTCCAGAGGCGTTAAC
ACAAGGTGACACCCGCCATGAGTCTCTCGAAGCCGCGCAGGGCGCATTAGTCACCGCCTTTGAATTTTATTTTGAAGATA
ACCAGCCAATTCCTTTACCTTCAATGGTTGATGAAGCTGATGATTATGTGGAGATTCCTCTAAGCATTGCGTCTAAAGTC
CTTTTGTTGAATGCGTTCCTTGAATCAAAAATCACGCAGCAGGAGCTGGCGAGGCGCATTGGCAGACCCAAGCAGGAAAT
TACCCGACTATTCGACTTGAAACACGCAACGAAAATCGATGCTGTACAGATTGCGGCCAGAGCATTGGGTAAAGAGTTAT
CGCTGACTATGCTTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1