Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 1745900..1747008 Replicon chromosome
Accession NZ_CP096522
Organism Staphylococcus aureus strain RIVM_M085090

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag M1F52_RS08440 Protein ID WP_002303392.1
Coordinates 1745900..1746769 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag M1F52_RS08445 Protein ID WP_002303393.1
Coordinates 1746784..1747008 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F52_RS08395 (M1F52_08375) 1740995..1741303 - 309 WP_000457386.1 50S ribosomal protein L21 -
M1F52_RS08400 (M1F52_08380) 1741790..1742320 - 531 WP_001259929.1 rod shape-determining protein MreD -
M1F52_RS08405 (M1F52_08385) 1742320..1743162 - 843 WP_000911033.1 rod shape-determining protein MreC -
M1F52_RS08410 (M1F52_08390) 1743360..1743479 - 120 WP_000840603.1 hypothetical protein -
M1F52_RS08415 (M1F52_08395) 1743553..1744026 - 474 WP_001236734.1 DUF4930 family protein -
M1F52_RS08420 (M1F52_08400) 1744186..1744383 + 198 WP_000025835.1 hypothetical protein -
M1F52_RS08425 (M1F52_08405) 1744500..1744784 - 285 WP_000171859.1 hypothetical protein -
M1F52_RS08430 (M1F52_08410) 1744852..1744998 - 147 Protein_1656 JAB domain-containing protein -
M1F52_RS08435 (M1F52_08415) 1745233..1745919 - 687 WP_224103254.1 class I SAM-dependent methyltransferase -
M1F52_RS08440 (M1F52_08420) 1745900..1746769 - 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
M1F52_RS08445 (M1F52_08425) 1746784..1747008 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
M1F52_RS08450 (M1F52_08430) 1747151..1747972 - 822 Protein_1660 recombinase zinc beta ribbon domain-containing protein -
M1F52_RS08455 (M1F52_08435) 1748660..1749463 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
M1F52_RS08460 (M1F52_08440) 1749517..1751001 - 1485 WP_074371031.1 ABC-F type ribosomal protection protein Lsa(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island lnu(B) / lsa(E) - 1744500..1752889 8389


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T243075 WP_002303392.1 NZ_CP096522:c1746769-1745900 [Staphylococcus aureus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T243075 NZ_OW969691:839547-839954 [Citrobacter koseri]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCACAATGGCTCTCTTTGCTCATTCATGGGCTTGTCGCCGC
ATTGGTTTTATTGATGCCCTGGCCATTGAGCTACACGCCGCTCTGGCTGATATTGCTTTCGCTGGTGGTGTTCGACTGTG
TGCGCAGTCAGCGGCGGATTAATACCCGGCAGGGCGAAATTAAACTGCTGATGGATGGGCGTTTACGTTGGCAAGGGCAA
GACTGGACGATTCAGGGGCCGCCTTGGATGCTTAAAACCGGCATGATGCTGCGTTTGCGCGCCGACAGCGGGAAGCGTCA
GCATTTGTGGCTGGCGGCAGACAGCATGGATGACGCCGAGTGGCGGGATTTACGGCGGTTAATGTTGCAACAGGCGACGC
AGGGGTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT243075 WP_002303393.1 NZ_CP096522:c1747008-1746784 [Staphylococcus aureus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT243075 NZ_OW969691:839300-839566 [Citrobacter koseri]
ATGGATATTAATAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTCGACATTTCTATCATGCCGTT
TTTCGAACATGAGTACGATAACTTAAGCGATGAAGAAAAATGCATCTTTGTTCGTTTGCTGGAATGCGACGATCCTGATT
TATTTAACTGGTTGATGAACCACGGTAAACCGGGGGATGCAGAACTGGAACAAATGGTGAAACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References