Detailed information of TA system TA024306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 493321..494158
• Replicon: chromosome
• Accession: NC_013361
• Organism: Escherichia coli O26:H11 str. 11368

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: ECO26_RS02370
• Protein ID: WP_000227784.1
• Coordinates: 493321..493863 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: ECO26_RS02375
• Protein ID: WP_001297137.1
• Coordinates: 493847..494158 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO26_RS02350	488860..489771	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
ECO26_RS02355	489939..490430	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
ECO26_RS02360	490558..491922	-	1365	WP_001000977.1	MFS transporter	-
ECO26_RS02365	492330..493265	+	936	WP_001375746.1	sel1 repeat family protein	-
ECO26_RS02370	493321..493863	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
ECO26_RS02375	493847..494158	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
ECO26_RS02380	494343..495233	-	891	WP_000971336.1	heme o synthase	-
ECO26_RS02385	495245..495574	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
ECO26_RS02390	495574..496188	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
ECO26_RS02395	496178..498169	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
ECO26_RS02400	498191..499138	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T24306 WP_000227784.1 NC_013361:c493863-493321 [Escherichia coli O26:H11 str. 11368]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T24306 NC_013361:c493863-493321 [Escherichia coli O26:H11 str. 11368]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT24306 WP_001297137.1 NC_013361:c494158-493847 [Escherichia coli O26:H11 str. 11368]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT24306 NC_013361:c494158-493847 [Escherichia coli O26:H11 str. 11368]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3