Detailed information of TA system TA243049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2733081..2733610
• Replicon: chromosome
• Accession: NZ_CP096272
• Organism: Staphylococcus aureus strain FJ0318

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M1E49_RS13510
• Protein ID: WP_000621175.1
• Coordinates: 2733248..2733610 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: M1E49_RS13505
• Protein ID: WP_271229945.1
• Coordinates: 2733081..2733251 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1E49_RS13475 (2728117)	2728117..2728677	+	561	WP_001092416.1	K(+)-transporting ATPase subunit C	-
M1E49_RS13480 (2728886)	2728886..2729365	+	480	WP_001287090.1	hypothetical protein	-
M1E49_RS13485 (2729358)	2729358..2730941	+	1584	WP_001294626.1	PH domain-containing protein	-
M1E49_RS13490 (2730928)	2730928..2731419	+	492	WP_001205910.1	PH domain-containing protein	-
M1E49_RS13495 (2731423)	2731423..2731782	+	360	WP_000581200.1	holo-ACP synthase	-
M1E49_RS13500 (2731848)	2731848..2732996	+	1149	WP_001281151.1	alanine racemase	-
M1E49_RS13505 (2733081)	2733081..2733251	+	171	WP_271229945.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M1E49_RS13510 (2733248)	2733248..2733610	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M1E49_RS13515 (2733959)	2733959..2734960	+	1002	WP_061839378.1	PP2C family protein-serine/threonine phosphatase	-
M1E49_RS13520 (2735079)	2735079..2735405	+	327	WP_001052491.1	anti-sigma factor antagonist	-
M1E49_RS13525 (2735407)	2735407..2735886	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
M1E49_RS13530 (2735861)	2735861..2736631	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T243049 WP_000621175.1 NZ_CP096272:2733248-2733610 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T243049 NZ_OW968419:40181-40711 [Enterobacter cloacae]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6274.81 Da
• Isoelectric Point: 4.0115

>AT243049 WP_271229945.1 NZ_CP096272:2733081-2733251 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSHSTNE

Nucleotide

• Length: 171 bp

>AT243049 NZ_OW968419:39908-40177 [Enterobacter cloacae]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA