Detailed information of TA system TA024304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 7664..8448
• Replicon: plasmid pZA1001
• Accession: NC_013356
• Organism: Zymomonas mobilis subsp. mobilis NCIMB 11163

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: ZA10_RS09480
• Protein ID: WP_012817669.1
• Coordinates: 7957..8448 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A806DAA8
• Locus tag: ZA10_RS09475
• Protein ID: WP_012817668.1
• Coordinates: 7664..7960 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ZA10_RS09440	3481..3744	-	264	WP_012817661.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
ZA10_RS09445	4195..4425	+	231	WP_012817662.1	hypothetical protein	-
ZA10_RS09450	4422..4802	+	381	WP_012817663.1	type II toxin-antitoxin system death-on-curing family toxin	-
ZA10_RS09455	5578..5814	+	237	WP_012817664.1	hypothetical protein	-
ZA10_RS09460	5811..6392	+	582	WP_012817665.1	zeta toxin family protein	-
ZA10_RS09465	6526..6786	-	261	WP_012817666.1	ribbon-helix-helix protein, CopG family	-
ZA10_RS09470	6816..7466	-	651	WP_012817667.1	AAA family ATPase	-
ZA10_RS09475	7664..7960	+	297	WP_012817668.1	DUF1778 domain-containing protein	Antitoxin
ZA10_RS09480	7957..8448	+	492	WP_012817669.1	GNAT family N-acetyltransferase	Toxin
ZA10_RS10145	8636..9405	+	770	Protein_14	IS5 family transposase	-
ZA10_RS09495	9553..9774	+	222	WP_148207755.1	transcriptional regulator	-
ZA10_RS10050	11593..11594	-	2	WP_148207756.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53380	53380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-97)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18011.05 Da
• Isoelectric Point: 9.9205

>T24304 WP_012817669.1 NC_013356:7957-8448 [Zymomonas mobilis subsp. mobilis NCIMB 11163]
VTITAPELLSPEHQVELFQSGVESLDLWLKKRALKNQMTGASRTFVACKNRRVMAYYALASSAITIKSAHGRFRRNMPDP
IPVVVLGRLAIDQSLHNQGVGRAMVRDAALRIIQAADTIGIRGMLVHALSDEAKSFYEHIGFEPSPIDPMMLMVTLTDLK
ANI

Nucleotide

• Length: 492 bp

>T24304 NC_013356:7957-8448 [Zymomonas mobilis subsp. mobilis NCIMB 11163]
GTGACAATAACAGCTCCAGAATTATTATCCCCAGAACATCAAGTTGAGTTATTTCAATCTGGTGTAGAGAGCCTTGATCT
ATGGCTGAAAAAGCGTGCTTTAAAAAATCAAATGACAGGAGCCTCTCGCACATTTGTAGCCTGTAAAAATCGACGGGTGA
TGGCCTATTATGCTCTGGCATCTAGCGCTATCACTATAAAATCAGCCCATGGACGATTTAGGCGTAATATGCCAGATCCT
ATTCCTGTTGTCGTTTTAGGGCGGCTTGCTATTGATCAATCTTTACATAACCAAGGCGTTGGACGAGCAATGGTAAGGGA
TGCAGCCTTAAGGATAATACAGGCAGCTGATACTATCGGAATTAGAGGTATGCTCGTTCATGCGCTATCTGATGAAGCCA
AATCTTTCTACGAACATATCGGTTTCGAACCGTCTCCAATAGACCCAATGATGTTGATGGTTACATTAACCGATTTAAAG
GCTAATATTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11269.03 Da
• Isoelectric Point: 9.8305

>AT24304 WP_012817668.1 NC_013356:7664-7960 [Zymomonas mobilis subsp. mobilis NCIMB 11163]
MVATTTISSPNKREALNIRIKPEERDLIDRAAKIQRKSRTDFVLEAACRAAEEALFDQLIITVDPKAYTNFLTRLDMPPR
PNDRLQKTMQTVAPWDKL

Nucleotide

• Length: 297 bp

>AT24304 NC_013356:7664-7960 [Zymomonas mobilis subsp. mobilis NCIMB 11163]
ATGGTAGCCACAACAACAATATCCTCACCAAACAAACGAGAAGCGCTGAATATTAGAATTAAACCGGAAGAACGTGATTT
AATAGATCGAGCGGCAAAAATTCAGAGAAAAAGCCGTACTGATTTTGTATTAGAGGCAGCTTGTAGGGCTGCAGAAGAAG
CACTGTTCGATCAATTAATCATTACTGTAGATCCCAAGGCCTATACTAATTTCTTAACACGCCTTGATATGCCACCACGC
CCAAATGATCGGCTGCAAAAAACCATGCAAACGGTAGCTCCATGGGATAAGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A806DAA8