Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 32529..33106 Replicon plasmid pKP20-558-4
Accession NZ_CP096267
Organism Klebsiella quasipneumoniae strain KP20-558

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag M0M92_RS29675 Protein ID WP_059065587.1
Coordinates 32774..33106 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag M0M92_RS29670 Protein ID WP_032454911.1
Coordinates 32529..32774 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0M92_RS29640 (M0M92_29650) 27638..29035 + 1398 WP_009653207.1 ISNCY family transposase -
M0M92_RS29645 (M0M92_29655) 29056..29247 + 192 Protein_33 hypothetical protein -
M0M92_RS29650 (M0M92_29660) 29275..29553 - 279 WP_004197671.1 hypothetical protein -
M0M92_RS29655 (M0M92_29665) 29888..30454 - 567 WP_009653205.1 LuxR C-terminal-related transcriptional regulator -
M0M92_RS29660 (M0M92_29670) 30586..30708 - 123 Protein_36 helix-turn-helix domain-containing protein -
M0M92_RS29665 (M0M92_29675) 30772..31476 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
M0M92_RS29670 (M0M92_29680) 32529..32774 + 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M0M92_RS29675 (M0M92_29685) 32774..33106 + 333 WP_059065587.1 endoribonuclease MazF Toxin
M0M92_RS29680 (M0M92_29690) 33181..33375 + 195 WP_071997591.1 hypothetical protein -
M0M92_RS29685 (M0M92_29695) 33372..33569 - 198 Protein_41 IS3 family transposase -
M0M92_RS29690 (M0M92_29700) 33846..34850 - 1005 WP_000427614.1 IS110-like element IS5075 family transposase -
M0M92_RS29695 (M0M92_29705) 34929..35363 - 435 WP_000429836.1 Hg(II)-responsive transcriptional regulator -
M0M92_RS29700 (M0M92_29710) 35435..35785 + 351 WP_001294663.1 mercuric transport protein MerT -
M0M92_RS29705 (M0M92_29715) 35799..36074 + 276 WP_000732292.1 mercury resistance system periplasmic binding protein MerP -
M0M92_RS29710 (M0M92_29720) 36110..36532 + 423 WP_001340589.1 organomercurial transporter MerC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / qacE / ere(A) / dfrA5 - 1..52802 52802
- inside IScluster/Tn - - 23522..34850 11328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T243033 WP_059065587.1 NZ_CP096267:32774-33106 [Klebsiella quasipneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

>T243033 NZ_OW968329:71972-72121 [Enterobacter cloacae]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT243033 WP_032454911.1 NZ_CP096267:32529-32774 [Klebsiella quasipneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp

>AT243033 NZ_OW968329:c71927-71868 [Enterobacter cloacae]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References