Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17825..18468 Replicon plasmid pKP20-558-4
Accession NZ_CP096267
Organism Klebsiella quasipneumoniae strain KP20-558

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag M0M92_RS29590 Protein ID WP_001044770.1
Coordinates 18052..18468 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag M0M92_RS29585 Protein ID WP_001261282.1
Coordinates 17825..18055 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0M92_RS29550 (M0M92_29560) 13397..14263 - 867 WP_023302476.1 replication initiation protein -
M0M92_RS29555 (M0M92_29565) 14798..14902 + 105 WP_032410267.1 hypothetical protein -
M0M92_RS29560 (M0M92_29570) 15031..15288 + 258 WP_000764642.1 hypothetical protein -
M0M92_RS29565 (M0M92_29575) 15346..16122 - 777 WP_023302478.1 site-specific integrase -
M0M92_RS29570 (M0M92_29580) 16119..16862 - 744 WP_004098973.1 hypothetical protein -
M0M92_RS29575 (M0M92_29585) 16913..17263 - 351 WP_000493378.1 hypothetical protein -
M0M92_RS29580 (M0M92_29590) 17407..17868 - 462 WP_179135310.1 hypothetical protein -
M0M92_RS29585 (M0M92_29595) 17825..18055 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M0M92_RS29590 (M0M92_29600) 18052..18468 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
M0M92_RS29595 (M0M92_29605) 18542..20104 + 1563 WP_004206609.1 AAA family ATPase -
M0M92_RS29600 (M0M92_29610) 20089..21111 + 1023 WP_000361404.1 helicase UvrD -
M0M92_RS29605 (M0M92_29615) 21655..22563 + 909 WP_080933653.1 HNH endonuclease -
M0M92_RS29610 (M0M92_29620) 22749..23099 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / qacE / ere(A) / dfrA5 - 1..52802 52802


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T243032 WP_001044770.1 NZ_CP096267:18052-18468 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T243032 NZ_OW968329:4188-4475 [Enterobacter cloacae]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTACTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGTGCCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTATGAACTTACTGGCCAGACTATTTGG
GGGCCGCATGGAATTCGGAAAATAAACCACGTTAAGACTATTTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT243032 WP_001261282.1 NZ_CP096267:17825-18055 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT243032 NZ_OW968329:3904-4188 [Enterobacter cloacae]
ATGCAAATGAAAAACAATACCGCACAAACAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCAGACAGCCTCAATACTGACAATCCGTTACCGGTGCCTCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References