Detailed information of TA system TA243031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 60845..61370
• Replicon: plasmid pKP20-558-3
• Accession: NZ_CP096266
• Organism: Klebsiella quasipneumoniae strain KP20-558

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: M0M92_RS28935
• Protein ID: WP_013023785.1
• Coordinates: 61065..61370 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: M0M92_RS28930
• Protein ID: WP_001568025.1
• Coordinates: 60845..61063 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0M92_RS28900 (M0M92_28910)	56335..57024	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
M0M92_RS28905 (M0M92_28915)	57056..57745	-	690	WP_004193995.1	hypothetical protein	-
M0M92_RS28910 (M0M92_28920)	58012..58893	+	882	WP_223289722.1	hypothetical protein	-
M0M92_RS28915 (M0M92_28925)	58995..59243	+	249	WP_053389907.1	hypothetical protein	-
M0M92_RS28920 (M0M92_28930)	59297..60283	-	987	WP_223289723.1	hypothetical protein	-
M0M92_RS28925 (M0M92_28935)	60421..60675	+	255	Protein_59	hypothetical protein	-
M0M92_RS28930 (M0M92_28940)	60845..61063	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
M0M92_RS28935 (M0M92_28945)	61065..61370	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
M0M92_RS28940 (M0M92_28950)	61539..61934	+	396	WP_162269742.1	hypothetical protein	-
M0M92_RS28945 (M0M92_28955)	61961..62275	+	315	WP_053389906.1	hypothetical protein	-
M0M92_RS28950 (M0M92_28960)	62286..63302	+	1017	WP_017899884.1	hypothetical protein	-
M0M92_RS28955 (M0M92_28965)	63500..64294	+	795	WP_053389905.1	site-specific integrase	-
M0M92_RS28960 (M0M92_28970)	64750..65052	-	303	WP_004197636.1	hypothetical protein	-
M0M92_RS28965 (M0M92_28975)	65049..65675	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..159894	159894

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T243031 WP_013023785.1 NZ_CP096266:61065-61370 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T243031 NZ_OW968328:c4814787-4814416 [Enterobacter cloacae]
ATGAATAATATGGCCGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTCGAAGCTGCGGATGCGATTGA
TCATGCCGCGTCACATCGAGCCATTAGCTTAATTACCTGGATGGAGGTGATGGTGGGCGCACGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTCGAAGTTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTGCGT
GAAAAGCATGGAATGAAACTGCCTGATGCCATCATTCTGGCCACAGCCCAGAGCAGAAAATGTCCGTTGATTTCTCGAAA
TACGAAAGATTTTGCGGGTATTGATGAGGTCGTTACGCCTTATCAACTGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT243031 WP_001568025.1 NZ_CP096266:60845-61063 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT243031 NZ_OW968328:c4815032-4814790 [Enterobacter cloacae]
ATGAGTGTTTTGGCGGAACTGGATATGGGCAGAATACTGATCGATTTATCTGATGACGTGATTCAACGGCTGGATAACCT
CAAGCAACTGCGAAATCAGCCGCGCGCTGAGTTGCTACGGGAGGCGATAGAGCTGTATCTCGACCAGCAGAGTTCGTCTG
TTATCCGCGATGCGCTTGGCTTGTGGGGAGACAAGAAAGAAGACGGGCTGGAGTATGAACGTAAGCTGCGTGAGGAATGG
TGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8