Detailed information of TA system TA243030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 33234..33970
• Replicon: plasmid pKP20-558-2
• Accession: NZ_CP096265
• Organism: Klebsiella quasipneumoniae strain KP20-558

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: M0M92_RS27805
• Protein ID: WP_209702800.1
• Coordinates: 33234..33716 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: M0M92_RS27810
• Protein ID: WP_248569408.1
• Coordinates: 33704..33970 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0M92_RS27775 (M0M92_27785)	28526..28675	+	150	Protein_25	transposase	-
M0M92_RS27780 (M0M92_27790)	28691..28801	+	111	Protein_26	IS3 family transposase	-
M0M92_RS27785 (M0M92_27795)	28953..29348	-	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
M0M92_RS27790 (M0M92_27800)	29397..30743	-	1347	WP_248569410.1	ISNCY family transposase	-
M0M92_RS27795 (M0M92_27805)	30969..31601	+	633	WP_001567369.1	hypothetical protein	-
M0M92_RS27800 (M0M92_27810)	31630..33033	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
M0M92_RS27805 (M0M92_27815)	33234..33716	-	483	WP_209702800.1	GNAT family N-acetyltransferase	Toxin
M0M92_RS27810 (M0M92_27820)	33704..33970	-	267	WP_248569408.1	DUF1778 domain-containing protein	Antitoxin
M0M92_RS27815 (M0M92_27825)	34208..34642	-	435	WP_032416767.1	cell envelope integrity protein TolA	-
M0M92_RS27820 (M0M92_27830)	34815..35840	+	1026	WP_087855116.1	IS110 family transposase	-
M0M92_RS27825 (M0M92_27835)	36135..37103	+	969	WP_248569404.1	IS5-like element IS903B family transposase	-
M0M92_RS27830 (M0M92_27840)	37156..37287	-	132	Protein_36	type I restriction-modification system subunit M N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..193803	193803
	-	inside	IScluster/Tn	-	-	27435..37103	9668

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17352.00 Da
• Isoelectric Point: 9.5822

>T243030 WP_209702800.1 NZ_CP096265:c33716-33234 [Klebsiella quasipneumoniae]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKVSQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T243030 NZ_OW968328:c4022080-4021589 [Enterobacter cloacae]
ATGATCTCTGCCCCAAAGCCCCTTCACGCCGGTCATATTCTTACTCCATTTTGCTGTGGTGTGGATTCGATGGATAACTG
GCTGAAGCAGCGGGCGATGAAAAATCAGGTCAGTGGTGCATCCCGTACCTTTGTCTGTTGTGGCAACGATTCTAACGTTC
TGGCTTATTACTCGCTGGCATCCAGTGCGGTCATGACGAATACCGCACCCGGTCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCGGTTGTTGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTGCATGGGCAGGGCGTTGGTCGGGCACTGGTACGTGA
CGCTGGGCTGCGGGTGATCCAGGTGGCGGAGACCATCGGCATTCGCGGAATGCTGGTTCATGCGCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCAATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGATG
GCGAGTATTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10148.58 Da
• Isoelectric Point: 4.4345

>AT243030 WP_248569408.1 NZ_CP096265:c33970-33704 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEEYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT243030 NZ_OW968328:c4022370-4022077 [Enterobacter cloacae]
ATGCCAGCTGCAAACACTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAGGCCTGCCGAGCGAGATCTTATCGA
TCGGGCGGCGAAAGCGAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCAGCCCGCGCTGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTGGCTCGTCTGGATCAGGCTCCAGCACCTAAT
GCCACACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAGCGGGAAAAATGA