Detailed information of TA system TA024303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 59242..59777
• Replicon: plasmid pO103
• Accession: NC_013354
• Organism: Escherichia coli O103:H2 str. 12009

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ECO103_RS28315
• Protein ID: WP_001270421.1
• Coordinates: 59242..59529 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: ECO103_RS28320
• Protein ID: WP_001132895.1
• Coordinates: 59526..59777 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO103_RS28285	54645..54824	-	180	WP_000526860.1	hypothetical protein	-
ECO103_RS28290	55027..55401	+	375	WP_001368887.1	hypothetical protein	-
ECO103_RS32020	55712..55873	-	162	Protein_49	epoxyqueuosine reductase QueH	-
ECO103_RS28695	55924..56064	+	141	Protein_50	serine/threonine protein kinase	-
ECO103_RS28300	56188..56928	+	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
ECO103_RS28305	57212..58189	-	978	WP_000361617.1	RepB family plasmid replication initiator protein	-
ECO103_RS31140	58835..59240	-	406	Protein_53	DDE-type integrase/transposase/recombinase	-
ECO103_RS28315	59242..59529	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECO103_RS28320	59526..59777	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ECO103_RS32100	59951..60124	-	174	WP_001435278.1	hypothetical protein	-
ECO103_RS28330	60495..61700	+	1206	WP_000817643.1	AAA family ATPase	-
ECO103_RS28335	61697..62659	+	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
ECO103_RS28340	62800..63962	-	1163	Protein_59	DUF4113 domain-containing protein	-
ECO103_RS28345	63843..64260	+	418	Protein_60	DUF1281 domain-containing protein	-
ECO103_RS28350	64264..64569	+	306	WP_042354182.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC	1..75546	75546

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T24303 WP_001270421.1 NC_013354:c59529-59242 [Escherichia coli O103:H2 str. 12009]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T24303 NC_013354:c59529-59242 [Escherichia coli O103:H2 str. 12009]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT24303 WP_001132895.1 NC_013354:c59777-59526 [Escherichia coli O103:H2 str. 12009]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT24303 NC_013354:c59777-59526 [Escherichia coli O103:H2 str. 12009]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTTTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6