Detailed information of TA system TA024302

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 5289561..5290336
• Replicon: chromosome
• Accession: NC_013353
• Organism: Escherichia coli O103:H2 str. 12009

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: ECO103_RS26995
• Protein ID: WP_001193489.1
• Coordinates: 5289965..5290336 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7V7JFU6
• Locus tag: ECO103_RS26990
• Protein ID: WP_001059301.1
• Coordinates: 5289561..5289926 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO103_RS26955	5285598..5286314	+	717	WP_000174910.1	WYL domain-containing protein	-
ECO103_RS26960	5286350..5286802	+	453	WP_000734322.1	hypothetical protein	-
ECO103_RS26965	5286874..5287347	+	474	WP_001298943.1	hypothetical protein	-
ECO103_RS26970	5287467..5288288	+	822	WP_001234408.1	DUF945 domain-containing protein	-
ECO103_RS26975	5288319..5288762	+	444	WP_001096016.1	antirestriction protein	-
ECO103_RS26980	5288775..5289317	+	543	WP_015740440.1	DNA repair protein RadC	-
ECO103_RS26985	5289314..5289535	+	222	WP_000691983.1	DUF987 domain-containing protein	-
ECO103_RS26990	5289561..5289926	+	366	WP_001059301.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECO103_RS26995	5289965..5290336	+	372	WP_001193489.1	TA system toxin CbtA family protein	Toxin
ECO103_RS27005	5291491..5292471	-	981	WP_000991444.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
ECO103_RS27010	5292536..5293642	-	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
ECO103_RS27015	5293662..5294378	-	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	5283510..5299905	16395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13875.97 Da
• Isoelectric Point: 7.6717

>T24302 WP_001193489.1 NC_013353:5289965-5290336 [Escherichia coli O103:H2 str. 12009]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYERVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Nucleotide

• Length: 372 bp

>T24302 NC_013353:5289965-5290336 [Escherichia coli O103:H2 str. 12009]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACGGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.9530

>AT24302 WP_001059301.1 NC_013353:5289561-5289926 [Escherichia coli O103:H2 str. 12009]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT24302 NC_013353:5289561-5289926 [Escherichia coli O103:H2 str. 12009]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7V7JFU6