Detailed information of TA system TA243019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2525429..2527017
• Replicon: chromosome
• Accession: NZ_CP096263
• Organism: Klebsiella quasipneumoniae strain KP20-558

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: M0M92_RS12080
• Protein ID: WP_044523012.1
• Coordinates: 2525695..2527017 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: M0M92_RS12075
• Protein ID: WP_023289667.1
• Coordinates: 2525429..2525695 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0M92_RS12050 (2520580)	2520580..2521503	+	924	WP_023289662.1	LysR family hpxDE operon transcriptional regulator HpxR	-
M0M92_RS12055 (2521484)	2521484..2522638	-	1155	WP_171879000.1	FAD-dependent urate hydroxylase HpxO	-
M0M92_RS12060 (2522984)	2522984..2524363	+	1380	WP_065812518.1	nucleobase:cation symporter-2 family protein	-
M0M92_RS12065 (2524341)	2524341..2524841	+	501	WP_032452825.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
M0M92_RS12070 (2524838)	2524838..2525164	+	327	WP_044523006.1	hydroxyisourate hydrolase	-
M0M92_RS12075 (2525429)	2525429..2525695	+	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
M0M92_RS12080 (2525695)	2525695..2527017	+	1323	WP_044523012.1	type II toxin-antitoxin system HipA family toxin	Toxin
M0M92_RS12085 (2527034)	2527034..2528326	-	1293	WP_044523014.1	glycoside hydrolase family 10 protein	-
M0M92_RS12090 (2528468)	2528468..2529472	-	1005	WP_044523017.1	fimbrial protein	-
M0M92_RS12095 (2529460)	2529460..2531979	-	2520	WP_101866108.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49183.49 Da
• Isoelectric Point: 8.6110

>T243019 WP_044523012.1 NZ_CP096263:2525695-2527017 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSAHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T243019 NZ_OW968289:101799-102197 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT243019 WP_023289667.1 NZ_CP096263:2525429-2525695 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT243019 NZ_OW968289:101572-101799 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3