Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1140903..1141522 Replicon chromosome
Accession NZ_CP096263
Organism Klebsiella quasipneumoniae strain KP20-558

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag M0M92_RS05380 Protein ID WP_002892050.1
Coordinates 1140903..1141121 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag M0M92_RS05385 Protein ID WP_002892066.1
Coordinates 1141148..1141522 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0M92_RS05345 (1136946) 1136946..1137209 + 264 WP_004204754.1 type B 50S ribosomal protein L31 -
M0M92_RS05350 (1137209) 1137209..1137349 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
M0M92_RS05355 (1137346) 1137346..1138044 - 699 WP_117128677.1 GNAT family protein -
M0M92_RS05360 (1138145) 1138145..1139602 + 1458 Protein_1026 PLP-dependent aminotransferase family protein -
M0M92_RS05365 (1139571) 1139571..1140041 - 471 WP_064155186.1 YlaC family protein -
M0M92_RS05370 (1140075) 1140075..1140206 + 132 WP_162543094.1 hypothetical protein -
M0M92_RS05375 (1140178) 1140178..1140744 - 567 WP_023288643.1 maltose O-acetyltransferase -
M0M92_RS05380 (1140903) 1140903..1141121 - 219 WP_002892050.1 HHA domain-containing protein Toxin
M0M92_RS05385 (1141148) 1141148..1141522 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
M0M92_RS05390 (1142007) 1142007..1145153 - 3147 WP_023288644.1 multidrug efflux RND transporter permease subunit AcrB -
M0M92_RS05395 (1145176) 1145176..1146369 - 1194 WP_023288645.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T243018 WP_002892050.1 NZ_CP096263:c1141121-1140903 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T243018 NZ_OW968288:c4390439-4390266 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT243018 WP_002892066.1 NZ_CP096263:c1141522-1141148 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT243018 NZ_OW968288:c4390763-4390467 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References