Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 150743..151395 Replicon plasmid pKP19-2796-1
Accession NZ_CP096261
Organism Klebsiella pneumoniae strain KP19-2796

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag M0M88_RS26720 Protein ID WP_017901321.1
Coordinates 150743..151168 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag M0M88_RS26725 Protein ID WP_001261275.1
Coordinates 151165..151395 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0M88_RS26685 (M0M88_26685) 146452..146568 + 117 Protein_155 transposase -
M0M88_RS26690 (M0M88_26690) 146693..146863 - 171 Protein_156 LysR family transcriptional regulator -
M0M88_RS26695 (M0M88_26695) 146874..147101 + 228 Protein_157 IS3 family transposase -
M0M88_RS26700 (M0M88_26700) 147193..148749 - 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
M0M88_RS26705 (M0M88_26705) 148936..149109 - 174 Protein_159 nuclease -
M0M88_RS26710 (M0M88_26710) 149162..149698 - 537 Protein_160 integrase core domain-containing protein -
M0M88_RS26715 (M0M88_26715) 149757..150725 - 969 WP_077254762.1 IS5 family transposase -
M0M88_RS26720 (M0M88_26720) 150743..151168 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
M0M88_RS26725 (M0M88_26725) 151165..151395 - 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M0M88_RS26730 (M0M88_26730) 151648..154224 - 2577 WP_017901322.1 nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(6)-Id / aph(3'')-Ib - 1..227518 227518
- flank IS/Tn - - 149757..150725 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T243015 WP_017901321.1 NZ_CP096261:c151168-150743 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T243015 NZ_OW968288:3792180-3792458 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT243015 WP_001261275.1 NZ_CP096261:c151395-151165 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT243015 NZ_OW968288:3791917-3792177 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References