Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5303876..5304501 Replicon chromosome
Accession NZ_CP096260
Organism Klebsiella pneumoniae strain KP19-2796

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M0M88_RS25545 Protein ID WP_002882817.1
Coordinates 5303876..5304259 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M0M88_RS25550 Protein ID WP_004150355.1
Coordinates 5304259..5304501 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0M88_RS25530 (5301242) 5301242..5302144 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M0M88_RS25535 (5302141) 5302141..5302776 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M0M88_RS25540 (5302773) 5302773..5303702 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M0M88_RS25545 (5303876) 5303876..5304259 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M0M88_RS25550 (5304259) 5304259..5304501 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M0M88_RS25555 (5304706) 5304706..5305623 + 918 WP_004181612.1 alpha/beta hydrolase -
M0M88_RS25560 (5305637) 5305637..5306578 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M0M88_RS25565 (5306623) 5306623..5307060 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M0M88_RS25570 (5307057) 5307057..5307917 - 861 WP_004146232.1 virulence factor BrkB family protein -
M0M88_RS25575 (5307911) 5307911..5308510 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T243012 WP_002882817.1 NZ_CP096260:c5304259-5303876 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T243012 NZ_OW968288:2704482-2704589 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT243012 WP_004150355.1 NZ_CP096260:c5304501-5304259 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT243012 NZ_OW968288:c2704435-2704369 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References