Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4062614..4063233 Replicon chromosome
Accession NZ_CP096260
Organism Klebsiella pneumoniae strain KP19-2796

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag M0M88_RS19750 Protein ID WP_002892050.1
Coordinates 4063015..4063233 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag M0M88_RS19745 Protein ID WP_002892066.1
Coordinates 4062614..4062988 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0M88_RS19735 (4057766) 4057766..4058959 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M0M88_RS19740 (4058982) 4058982..4062128 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
M0M88_RS19745 (4062614) 4062614..4062988 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
M0M88_RS19750 (4063015) 4063015..4063233 + 219 WP_002892050.1 HHA domain-containing protein Toxin
M0M88_RS19755 (4063392) 4063392..4063958 + 567 WP_002892030.1 maltose O-acetyltransferase -
M0M88_RS19760 (4063930) 4063930..4064070 - 141 WP_004147370.1 hypothetical protein -
M0M88_RS19765 (4064091) 4064091..4064561 + 471 WP_002892026.1 YlaC family protein -
M0M88_RS19770 (4064536) 4064536..4065987 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
M0M88_RS19775 (4066088) 4066088..4066786 + 699 WP_002892021.1 GNAT family protein -
M0M88_RS19780 (4066783) 4066783..4066923 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
M0M88_RS19785 (4066923) 4066923..4067186 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T243008 WP_002892050.1 NZ_CP096260:4063015-4063233 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T243008 NZ_OW968288:2295214-2295501 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT243008 WP_002892066.1 NZ_CP096260:4062614-4062988 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT243008 NZ_OW968288:2294975-2295214 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References