Detailed information of TA system TA024300

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5074889..5075687
• Replicon: chromosome
• Accession: NC_013353
• Organism: Escherichia coli O103:H2 str. 12009

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0F3UPG9
• Locus tag: ECO103_RS25935
• Protein ID: WP_000854698.1
• Coordinates: 5074889..5075266 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0F3T7G0
• Locus tag: ECO103_RS25940
• Protein ID: WP_000017094.1
• Coordinates: 5075313..5075687 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO103_RS25910	5070981..5072519	-	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-
ECO103_RS25920	5073268..5074110	-	843	WP_001280439.1	DUF4942 domain-containing protein	-
ECO103_RS25925	5074195..5074392	-	198	WP_000839251.1	DUF957 domain-containing protein	-
ECO103_RS25930	5074404..5074892	-	489	WP_001054226.1	hypothetical protein	-
ECO103_RS25935	5074889..5075266	-	378	WP_000854698.1	TA system toxin CbtA family protein	Toxin
ECO103_RS25940	5075313..5075687	-	375	WP_000017094.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECO103_RS25945	5075762..5075983	-	222	WP_001220316.1	DUF987 domain-containing protein	-
ECO103_RS25950	5076052..5076528	-	477	WP_001186196.1	RadC family protein	-
ECO103_RS25955	5076543..5077028	-	486	WP_000213700.1	antirestriction protein	-
ECO103_RS25960	5077119..5077937	-	819	WP_001234727.1	DUF945 domain-containing protein	-
ECO103_RS25965	5078092..5078250	-	159	WP_001369071.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13975.94 Da
• Isoelectric Point: 7.9087

>T24300 WP_000854698.1 NC_013353:c5075266-5074889 [Escherichia coli O103:H2 str. 12009]
MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T24300 NC_013353:c5075266-5074889 [Escherichia coli O103:H2 str. 12009]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13732.42 Da
• Isoelectric Point: 5.4488

>AT24300 WP_000017094.1 NC_013353:c5075687-5075313 [Escherichia coli O103:H2 str. 12009]
VSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT24300 NC_013353:c5075687-5075313 [Escherichia coli O103:H2 str. 12009]
GTGTCAGGCACACTCCATGAGACAAATTATCCCAACGACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAATTACATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0F3UPG9

Antitoxin

• AlphaFold DB: A0A0F3T7G0