Detailed information of TA system TA242981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 7594421..7594992
• Replicon: chromosome
• Accession: NZ_CP096255
• Organism: Bradyrhizobium barranii subsp. apii strain 1S5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HAP41_RS36470
• Protein ID: WP_166084753.1
• Coordinates: 7594639..7594992 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8T5V1Z1
• Locus tag: HAP41_RS36465
• Protein ID: WP_063985199.1
• Coordinates: 7594421..7594642 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP41_RS36455 (HAP41_0000036455)	7589821..7592958	-	3138	WP_166084763.1	efflux RND transporter permease subunit	-
HAP41_RS36460 (HAP41_0000036460)	7593135..7594241	-	1107	WP_135216330.1	efflux RND transporter periplasmic adaptor subunit	-
HAP41_RS36465 (HAP41_0000036465)	7594421..7594642	+	222	WP_063985199.1	antitoxin MazE family protein	Antitoxin
HAP41_RS36470 (HAP41_0000036470)	7594639..7594992	+	354	WP_166084753.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HAP41_RS36475 (HAP41_0000036475)	7595079..7596409	-	1331	Protein_7276	MFS transporter	-
HAP41_RS36480 (HAP41_0000036480)	7596489..7596917	+	429	WP_166084740.1	DUF2809 domain-containing protein	-
HAP41_RS36485 (HAP41_0000036485)	7597041..7598204	-	1164	WP_166084738.1	crosslink repair DNA glycosylase YcaQ family protein	-
HAP41_RS36490 (HAP41_0000036490)	7598386..7599729	+	1344	WP_166053720.1	IS1380 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	7598386..7599729	1343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin MazE-like (11-73)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12574.33 Da
• Isoelectric Point: 6.3275

>T242981 WP_166084753.1 NZ_CP096255:7594639-7594992 [Bradyrhizobium barranii subsp. apii]
MKRGEVWTAAGGKDYAGKPRPVVIVQDDSFDATNSITVCAFTTDETEAPLFRLKVEPNDGNGLRAPSRLMVDKITTVPKA
KVGTLVGRLDDDDLLRLNRAILVFLGLAGSQRSASAD

Nucleotide

• Length: 354 bp

>T242981 NZ_OW968277:3188173-3188280 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8284.21 Da
• Isoelectric Point: 7.3202

>AT242981 WP_063985199.1 NZ_CP096255:7594421-7594642 [Bradyrhizobium barranii subsp. apii]
MSPSKPKPSRMKVQAHRDRLRAQGLRPIQIWVPDIRSPSFQSEAHRQSLAVATSSHESEDQGFIDAVSDWTDE

Nucleotide

• Length: 222 bp

>AT242981 NZ_OW968277:c3188124-3188059 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT