Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 1774438..1775009 Replicon chromosome
Accession NZ_CP096251
Organism Bradyrhizobium barranii subsp. apii strain 38S5

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag J4G48_RS08325 Protein ID WP_166084753.1
Coordinates 1774438..1774791 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A8T5V1Z1
Locus tag J4G48_RS08330 Protein ID WP_063985199.1
Coordinates 1774788..1775009 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4G48_RS08305 (J4G48_0008305) 1770756..1771001 + 246 WP_166084715.1 hypothetical protein -
J4G48_RS08310 (J4G48_0008310) 1771234..1772397 + 1164 WP_208073593.1 crosslink repair DNA glycosylase YcaQ family protein -
J4G48_RS08315 (J4G48_0008315) 1772512..1772940 - 429 WP_166084740.1 DUF2809 domain-containing protein -
J4G48_RS08320 (J4G48_0008320) 1773020..1774351 + 1332 WP_208073592.1 MFS transporter -
J4G48_RS08325 (J4G48_0008325) 1774438..1774791 - 354 WP_166084753.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J4G48_RS08330 (J4G48_0008330) 1774788..1775009 - 222 WP_063985199.1 antitoxin MazE family protein Antitoxin
J4G48_RS08335 (J4G48_0008335) 1775189..1776295 + 1107 WP_135216330.1 efflux RND transporter periplasmic adaptor subunit -
J4G48_RS08340 (J4G48_0008340) 1776472..1779609 + 3138 WP_208073591.1 efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-107)

Antitoxin

Antitoxin MazE-like

(11-73)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 12574.33 Da        Isoelectric Point: 6.3275

>T242977 WP_166084753.1 NZ_CP096251:c1774791-1774438 [Bradyrhizobium barranii subsp. apii]
MKRGEVWTAAGGKDYAGKPRPVVIVQDDSFDATNSITVCAFTTDETEAPLFRLKVEPNDGNGLRAPSRLMVDKITTVPKA
KVGTLVGRLDDDDLLRLNRAILVFLGLAGSQRSASAD

Download         Length: 354 bp

>T242977 NZ_OW968277:c2257753-2257403 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8284.21 Da        Isoelectric Point: 7.3202

>AT242977 WP_063985199.1 NZ_CP096251:c1775009-1774788 [Bradyrhizobium barranii subsp. apii]
MSPSKPKPSRMKVQAHRDRLRAQGLRPIQIWVPDIRSPSFQSEAHRQSLAVATSSHESEDQGFIDAVSDWTDE

Download         Length: 222 bp

>AT242977 NZ_OW968277:c2257998-2257747 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References