Detailed information of TA system TA242976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 5959..6539
• Replicon: plasmid pDD01665-4
• Accession: NZ_CP096245
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01665

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: M0535_RS28075
• Protein ID: WP_071177730.1
• Coordinates: 6225..6539 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: M0535_RS28070
• Protein ID: WP_000093040.1
• Coordinates: 5959..6237 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0535_RS28040 (M0535_28000)	2019..2168	-	150	WP_032440454.1	colicin release lysis protein	-
M0535_RS28045 (M0535_28005)	2253..2510	-	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
M0535_RS28050 (M0535_28010)	2520..4205	-	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
M0535_RS28055 (M0535_28015)	4532..4777	+	246	WP_032440458.1	hypothetical protein	-
M0535_RS28060 (M0535_28020)	5051..5422	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
M0535_RS28065 (M0535_28025)	5419..5784	+	366	WP_072354022.1	TonB family protein	-
M0535_RS28070 (M0535_28030)	5959..6237	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
M0535_RS28075 (M0535_28035)	6225..6539	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M0535_RS28080 (M0535_28040)	6703..7131	+	429	WP_001140599.1	hypothetical protein	-
M0535_RS28085 (M0535_28045)	7157..7336	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
M0535_RS28090 (M0535_28050)	7363..7893	-	531	WP_071177729.1	hypothetical protein	-
M0535_RS28095 (M0535_28055)	7900..8631	-	732	WP_071177728.1	MobC family replication-relaxation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T242976 WP_071177730.1 NZ_CP096245:6225-6539 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T242976 NZ_OW968277:c2160728-2160351 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT242976 WP_000093040.1 NZ_CP096245:5959-6237 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT242976 NZ_OW968277:c2161186-2160818 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1