Detailed information of TA system TA242975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 7025..7668
• Replicon: plasmid pKPC-DD01665
• Accession: NZ_CP096244
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01665

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: M0535_RS27535
• Protein ID: WP_001044770.1
• Coordinates: 7252..7668 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: M0535_RS27530
• Protein ID: WP_001261282.1
• Coordinates: 7025..7255 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0535_RS27490 (M0535_27455)	2174..2476	+	303	WP_004197636.1	hypothetical protein	-
M0535_RS27495 (M0535_27460)	2988..3782	-	795	WP_004197635.1	site-specific integrase	-
M0535_RS27500 (M0535_27465)	3980..4996	-	1017	WP_017899884.1	hypothetical protein	-
M0535_RS27505 (M0535_27470)	5007..5321	-	315	WP_053389906.1	hypothetical protein	-
M0535_RS27510 (M0535_27475)	5348..5743	-	396	WP_017899885.1	hypothetical protein	-
M0535_RS27515 (M0535_27480)	5912..6217	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
M0535_RS27520 (M0535_27485)	6219..6437	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
M0535_RS27525 (M0535_27490)	6607..7068	-	462	WP_160880186.1	hypothetical protein	-
M0535_RS27530 (M0535_27495)	7025..7255	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M0535_RS27535 (M0535_27500)	7252..7668	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
M0535_RS27540 (M0535_27505)	7742..9304	+	1563	WP_004206609.1	AAA family ATPase	-
M0535_RS27545 (M0535_27510)	9289..10311	+	1023	WP_000361404.1	helicase UvrD	-
M0535_RS27550 (M0535_27515)	10567..11264	+	698	WP_223174630.1	IS1 family transposase	-
M0535_RS27555 (M0535_27520)	11293..11565	+	273	Protein_15	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..94000	94000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T242975 WP_001044770.1 NZ_CP096244:7252-7668 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T242975 NZ_OW968277:c2077535-2077248 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT242975 WP_001261282.1 NZ_CP096244:7025-7255 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT242975 NZ_OW968277:c2077261-2076959 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3