242970

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5382596..5383221 Replicon chromosome
Accession NZ_CP096242
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01665

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag M0535_RS25875 Protein ID WP_012737091.1
Coordinates 5382596..5382979 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M0535_RS25880 Protein ID WP_004150355.1
Coordinates 5382979..5383221 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0535_RS25860 (5379962) 5379962..5380864 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M0535_RS25865 (5380861) 5380861..5381496 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M0535_RS25870 (5381493) 5381493..5382422 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M0535_RS25875 (5382596) 5382596..5382979 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
M0535_RS25880 (5382979) 5382979..5383221 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M0535_RS25885 (5383426) 5383426..5384343 + 918 WP_012737090.1 alpha/beta hydrolase -
M0535_RS25890 (5384357) 5384357..5385298 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M0535_RS25895 (5385343) 5385343..5385780 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M0535_RS25900 (5385777) 5385777..5386637 - 861 WP_004146232.1 virulence factor BrkB family protein -
M0535_RS25905 (5386631) 5386631..5387230 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T242970 WP_012737091.1 NZ_CP096242:c5382979-5382596 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T242970 NZ_OW968277:c846174-845923 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT242970 WP_004150355.1 NZ_CP096242:c5383221-5382979 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT242970 NZ_OW968277:c845926-845672 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References