Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4292310..4292941 Replicon chromosome
Accession NC_013353
Organism Escherichia coli O103:H2 str. 12009

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag ECO103_RS22220 Protein ID WP_001259385.1
Coordinates 4292666..4292941 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag ECO103_RS22215 Protein ID WP_000593554.1
Coordinates 4292310..4292669 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO103_RS22185 4287422..4287895 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
ECO103_RS22190 4287892..4288173 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
ECO103_RS22195 4288250..4289608 + 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
ECO103_RS22200 4289601..4291109 + 1509 WP_001298718.1 carbohydrate kinase -
ECO103_RS22205 4291099..4291368 + 270 WP_000933060.1 HPr family phosphocarrier protein -
ECO103_RS22210 4291400..4292260 + 861 WP_001131783.1 class II aldolase -
ECO103_RS22215 4292310..4292669 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECO103_RS22220 4292666..4292941 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
ECO103_RS22225 4293014..4294138 - 1125 WP_001216257.1 ABC transporter permease -
ECO103_RS22230 4294138..4296873 - 2736 WP_000149167.1 ribosome-associated ATPase/putative transporter RbbA -
ECO103_RS22235 4296870..4297937 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T24297 WP_001259385.1 NC_013353:c4292941-4292666 [Escherichia coli O103:H2 str. 12009]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T24297 NC_013353:c4292941-4292666 [Escherichia coli O103:H2 str. 12009]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT24297 WP_000593554.1 NC_013353:c4292669-4292310 [Escherichia coli O103:H2 str. 12009]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT24297 NC_013353:c4292669-4292310 [Escherichia coli O103:H2 str. 12009]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References