Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4154159..4154778 Replicon chromosome
Accession NZ_CP096242
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01665

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag M0535_RS20055 Protein ID WP_002892050.1
Coordinates 4154560..4154778 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag M0535_RS20050 Protein ID WP_002892066.1
Coordinates 4154159..4154533 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0535_RS20040 (4149311) 4149311..4150504 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M0535_RS20045 (4150527) 4150527..4153673 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
M0535_RS20050 (4154159) 4154159..4154533 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
M0535_RS20055 (4154560) 4154560..4154778 + 219 WP_002892050.1 HHA domain-containing protein Toxin
M0535_RS20060 (4154937) 4154937..4155503 + 567 WP_002892030.1 maltose O-acetyltransferase -
M0535_RS20065 (4155475) 4155475..4155615 - 141 WP_004147370.1 hypothetical protein -
M0535_RS20070 (4155636) 4155636..4156106 + 471 WP_002892026.1 YlaC family protein -
M0535_RS20075 (4156081) 4156081..4157532 - 1452 WP_270104319.1 PLP-dependent aminotransferase family protein -
M0535_RS20080 (4157633) 4157633..4158331 + 699 WP_002892021.1 GNAT family protein -
M0535_RS20085 (4158328) 4158328..4158468 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
M0535_RS20090 (4158468) 4158468..4158731 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T242967 WP_002892050.1 NZ_CP096242:4154560-4154778 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T242967 NZ_OW968277:c515803-515701 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT242967 WP_002892066.1 NZ_CP096242:4154159-4154533 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT242967 NZ_OW968277:515663-515931 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References