Detailed information of TA system TA242953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 157661..158331
• Replicon: plasmid pVirDD02201
• Accession: NZ_CP096239
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02201

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: M0536_RS27540
• Protein ID: WP_004213072.1
• Coordinates: 157661..158104 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: M0536_RS27545
• Protein ID: WP_004213073.1
• Coordinates: 158101..158331 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0536_RS27505 (M0536_27460)	153072..153347	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
M0536_RS27510 (M0536_27465)	153410..153901	+	492	WP_011251327.1	DM13 domain-containing protein	-
M0536_RS27515 (M0536_27470)	153950..154870	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
M0536_RS27520 (M0536_27475)	154961..155364	+	404	Protein_168	GAF domain-containing protein	-
M0536_RS27525 (M0536_27480)	155882..156517	-	636	Protein_169	mucoid phenotype regulator RmpA2	-
M0536_RS27530 (M0536_27485)	156934..157238	+	305	Protein_170	transposase	-
M0536_RS27535 (M0536_27490)	157261..157512	-	252	WP_186987481.1	hypothetical protein	-
M0536_RS27540 (M0536_27495)	157661..158104	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
M0536_RS27545 (M0536_27500)	158101..158331	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M0536_RS27550 (M0536_27505)	158939..160072	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
M0536_RS27555 (M0536_27510)	160088..160381	+	294	WP_004213076.1	hypothetical protein	-
M0536_RS27560 (M0536_27515)	160371..160577	-	207	WP_004213077.1	hypothetical protein	-
M0536_RS27565 (M0536_27520)	160929..161219	+	291	WP_004213078.1	hypothetical protein	-
M0536_RS27570 (M0536_27525)	161209..162108	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..234915	234915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T242953 WP_004213072.1 NZ_CP096239:c158104-157661 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T242953 NZ_OW968124:c2508235-2508059 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT242953 WP_004213073.1 NZ_CP096239:c158331-158101 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT242953 NZ_OW968124:c2508013-2507597 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620