Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5455724..5456349 Replicon chromosome
Accession NZ_CP096238
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02201

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag M0536_RS26315 Protein ID WP_012737091.1
Coordinates 5455724..5456107 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M0536_RS26320 Protein ID WP_004150355.1
Coordinates 5456107..5456349 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0536_RS26300 (5453090) 5453090..5453992 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M0536_RS26305 (5453989) 5453989..5454624 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M0536_RS26310 (5454621) 5454621..5455550 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M0536_RS26315 (5455724) 5455724..5456107 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
M0536_RS26320 (5456107) 5456107..5456349 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M0536_RS26325 (5456554) 5456554..5457471 + 918 WP_012737090.1 alpha/beta hydrolase -
M0536_RS26330 (5457485) 5457485..5458426 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M0536_RS26335 (5458471) 5458471..5458908 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M0536_RS26340 (5458905) 5458905..5459765 - 861 WP_004146232.1 virulence factor BrkB family protein -
M0536_RS26345 (5459759) 5459759..5460358 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T242950 WP_012737091.1 NZ_CP096238:c5456107-5455724 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T242950 NZ_OW968124:2007104-2007207 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT242950 WP_004150355.1 NZ_CP096238:c5456349-5456107 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT242950 NZ_OW968124:c2007245-2006974 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References