Detailed information of TA system TA024295

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3828449..3829286
• Replicon: chromosome
• Accession: NC_013353
• Organism: Escherichia coli O103:H2 str. 12009

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: ECO103_RS19790
• Protein ID: WP_000854687.1
• Coordinates: 3828449..3828829 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ECO103_RS19795
• Protein ID: WP_012816826.1
• Coordinates: 3828918..3829286 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO103_RS19760	3824091..3825932	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
ECO103_RS19765	3826011..3826517	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
ECO103_RS19775	3826817..3827662	-	846	WP_001274562.1	DUF4942 domain-containing protein	-
ECO103_RS19780	3827747..3827944	-	198	WP_000772026.1	DUF957 domain-containing protein	-
ECO103_RS19785	3827964..3828452	-	489	WP_001054227.1	hypothetical protein	-
ECO103_RS19790	3828449..3828829	-	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
ECO103_RS19795	3828918..3829286	-	369	WP_012816826.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECO103_RS19800	3829361..3829582	-	222	WP_000692340.1	DUF987 domain-containing protein	-
ECO103_RS19805	3829651..3830127	-	477	WP_001364966.1	RadC family protein	-
ECO103_RS19810	3830143..3830616	-	474	WP_001364892.1	antirestriction protein	-
ECO103_RS19815	3830710..3830955	-	246	WP_000680588.1	hypothetical protein	-
ECO103_RS19820	3830955..3831776	-	822	WP_001234566.1	DUF945 domain-containing protein	-
ECO103_RS19825	3831996..3832406	-	411	WP_000848830.1	hypothetical protein	-
ECO103_RS19830	3832422..3833099	-	678	WP_000775503.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T24295 WP_000854687.1 NC_013353:c3828829-3828449 [Escherichia coli O103:H2 str. 12009]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T24295 NC_013353:c3828829-3828449 [Escherichia coli O103:H2 str. 12009]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13780.56 Da
• Isoelectric Point: 6.4763

>AT24295 WP_012816826.1 NC_013353:c3829286-3828918 [Escherichia coli O103:H2 str. 12009]
VSDTLHETNYPNDNHNRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT24295 NC_013353:c3829286-3828918 [Escherichia coli O103:H2 str. 12009]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACAACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67