Detailed information of TA system TA242948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4815703..4816513
• Replicon: chromosome
• Accession: NZ_CP096238
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02201

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: M0536_RS23335
• Protein ID: WP_270104284.1
• Coordinates: 4815703..4816236 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: M0536_RS23340
• Protein ID: WP_002887278.1
• Coordinates: 4816247..4816513 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0536_RS23330 (4814534)	4814534..4815655	+	1122	WP_012737335.1	cupin domain-containing protein	-
M0536_RS23335 (4815703)	4815703..4816236	-	534	WP_270104284.1	type II toxin-antitoxin system toxin KacT	Toxin
M0536_RS23340 (4816247)	4816247..4816513	-	267	WP_002887278.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
M0536_RS23345 (4816616)	4816616..4818049	-	1434	WP_014908043.1	Cu(+)/Ag(+) sensor histidine kinase	-
M0536_RS23350 (4818039)	4818039..4818722	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
M0536_RS23355 (4818894)	4818894..4820279	+	1386	WP_012737332.1	efflux transporter outer membrane subunit	-
M0536_RS23360 (4820297)	4820297..4820641	+	345	WP_012737331.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19825.70 Da
• Isoelectric Point: 5.2614

>T242948 WP_270104284.1 NZ_CP096238:c4816236-4815703 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVETLNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T242948 NZ_OW968124:1794918-1795169 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT242948 WP_002887278.1 NZ_CP096238:c4816513-4816247 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT242948 NZ_OW968124:1795166-1795420 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1