Detailed information of TA system TA024294

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3671928..3672726
• Replicon: chromosome
• Accession: NC_013353
• Organism: Escherichia coli O103:H2 str. 12009

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0F3UPG9
• Locus tag: ECO103_RS18935
• Protein ID: WP_000854698.1
• Coordinates: 3672349..3672726 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0F3T7G0
• Locus tag: ECO103_RS18930
• Protein ID: WP_000017094.1
• Coordinates: 3671928..3672302 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO103_RS18895	3667007..3668220	+	1214	WP_162860177.1	IS3 family transposase	-
ECO103_RS18900	3668263..3669294	+	1032	Protein_3589	autotransporter domain-containing protein	-
ECO103_RS18905	3669365..3669523	+	159	WP_001369071.1	DUF905 family protein	-
ECO103_RS18910	3669678..3670496	+	819	WP_001234727.1	DUF945 domain-containing protein	-
ECO103_RS18915	3670587..3671072	+	486	WP_000213700.1	antirestriction protein	-
ECO103_RS18920	3671087..3671563	+	477	WP_001186195.1	RadC family protein	-
ECO103_RS18925	3671632..3671853	+	222	WP_001220316.1	DUF987 domain-containing protein	-
ECO103_RS18930	3671928..3672302	+	375	WP_000017094.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECO103_RS18935	3672349..3672726	+	378	WP_000854698.1	TA system toxin CbtA family protein	Toxin
ECO103_RS18940	3672723..3673211	+	489	WP_001054226.1	hypothetical protein	-
ECO103_RS18945	3673223..3673420	+	198	WP_000839251.1	DUF957 domain-containing protein	-
ECO103_RS18950	3673505..3674347	+	843	WP_001280439.1	DUF4942 domain-containing protein	-
ECO103_RS18960	3675550..3676914	-	1365	WP_000953522.1	EspK/GogB family type III secretion system effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13975.94 Da
• Isoelectric Point: 7.9087

>T24294 WP_000854698.1 NC_013353:3672349-3672726 [Escherichia coli O103:H2 str. 12009]
MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T24294 NC_013353:3672349-3672726 [Escherichia coli O103:H2 str. 12009]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13732.42 Da
• Isoelectric Point: 5.4488

>AT24294 WP_000017094.1 NC_013353:3671928-3672302 [Escherichia coli O103:H2 str. 12009]
VSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT24294 NC_013353:3671928-3672302 [Escherichia coli O103:H2 str. 12009]
GTGTCAGGCACACTCCATGAGACAAATTATCCCAACGACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAATTACATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0F3UPG9

Antitoxin

• AlphaFold DB: A0A0F3T7G0