Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6985..7628 Replicon plasmid pKPC-DD02357
Accession NZ_CP096236
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02357

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag M0537_RS27600 Protein ID WP_001044770.1
Coordinates 7212..7628 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag M0537_RS27595 Protein ID WP_001261282.1
Coordinates 6985..7215 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0537_RS27555 (2174) 2174..2476 + 303 WP_004197636.1 hypothetical protein -
M0537_RS27560 (2948) 2948..3742 - 795 WP_004197635.1 site-specific integrase -
M0537_RS27565 (3940) 3940..4956 - 1017 WP_017899884.1 hypothetical protein -
M0537_RS27570 (4967) 4967..5281 - 315 WP_053389906.1 hypothetical protein -
M0537_RS27575 (5308) 5308..5703 - 396 WP_017899885.1 hypothetical protein -
M0537_RS27580 (5872) 5872..6177 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
M0537_RS27585 (6179) 6179..6397 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
M0537_RS27590 (6567) 6567..7028 - 462 WP_160866775.1 hypothetical protein -
M0537_RS27595 (6985) 6985..7215 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M0537_RS27600 (7212) 7212..7628 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
M0537_RS27605 (7702) 7702..9264 + 1563 WP_004206609.1 AAA family ATPase -
M0537_RS27610 (9249) 9249..10271 + 1023 WP_000361404.1 helicase UvrD -
M0537_RS27615 (10527) 10527..11224 + 698 WP_223174630.1 IS1 family transposase -
M0537_RS27620 (11253) 11253..11525 + 273 Protein_15 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / qnrS1 - 1..103167 103167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T242935 WP_001044770.1 NZ_CP096236:7212-7628 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T242935 NZ_OW968111:3487639-3487857 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT242935 WP_001261282.1 NZ_CP096236:6985-7215 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT242935 NZ_OW968111:3487239-3487613 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References