Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5872..6397 Replicon plasmid pKPC-DD02357
Accession NZ_CP096236
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02357

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag M0537_RS27580 Protein ID WP_013023785.1
Coordinates 5872..6177 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag M0537_RS27585 Protein ID WP_001568025.1
Coordinates 6179..6397 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0537_RS27550 (1551) 1551..2177 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
M0537_RS27555 (2174) 2174..2476 + 303 WP_004197636.1 hypothetical protein -
M0537_RS27560 (2948) 2948..3742 - 795 WP_004197635.1 site-specific integrase -
M0537_RS27565 (3940) 3940..4956 - 1017 WP_017899884.1 hypothetical protein -
M0537_RS27570 (4967) 4967..5281 - 315 WP_053389906.1 hypothetical protein -
M0537_RS27575 (5308) 5308..5703 - 396 WP_017899885.1 hypothetical protein -
M0537_RS27580 (5872) 5872..6177 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
M0537_RS27585 (6179) 6179..6397 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
M0537_RS27590 (6567) 6567..7028 - 462 WP_160866775.1 hypothetical protein -
M0537_RS27595 (6985) 6985..7215 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
M0537_RS27600 (7212) 7212..7628 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
M0537_RS27605 (7702) 7702..9264 + 1563 WP_004206609.1 AAA family ATPase -
M0537_RS27610 (9249) 9249..10271 + 1023 WP_000361404.1 helicase UvrD -
M0537_RS27615 (10527) 10527..11224 + 698 WP_223174630.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / qnrS1 - 1..103167 103167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T242934 WP_013023785.1 NZ_CP096236:c6177-5872 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242934 NZ_OW968111:2672338-2672445 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT242934 WP_001568025.1 NZ_CP096236:c6397-6179 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242934 NZ_OW968111:c2672288-2672225 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References