Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 156014..156684 Replicon plasmid pVirDD02357
Accession NZ_CP096235
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02357

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag M0537_RS27155 Protein ID WP_004213072.1
Coordinates 156014..156457 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag M0537_RS27160 Protein ID WP_004213073.1
Coordinates 156454..156684 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0537_RS27120 (M0537_27085) 151423..151698 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
M0537_RS27125 (M0537_27090) 151761..152252 + 492 WP_011251327.1 DM13 domain-containing protein -
M0537_RS27130 (M0537_27095) 152301..153221 + 921 WP_004213934.1 DUF1471 domain-containing protein -
M0537_RS27135 (M0537_27100) 153312..153715 + 404 Protein_168 GAF domain-containing protein -
M0537_RS27140 (M0537_27105) 154233..154870 - 638 Protein_169 mucoid phenotype regulator RmpA2 -
M0537_RS27145 (M0537_27110) 155287..155591 + 305 Protein_170 transposase -
M0537_RS27150 (M0537_27115) 155614..155865 - 252 WP_186987481.1 hypothetical protein -
M0537_RS27155 (M0537_27120) 156014..156457 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
M0537_RS27160 (M0537_27125) 156454..156684 - 231 WP_004213073.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M0537_RS27165 (M0537_27130) 157292..158425 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
M0537_RS27170 (M0537_27135) 158441..158734 + 294 WP_004213076.1 hypothetical protein -
M0537_RS27175 (M0537_27140) 158724..158930 - 207 WP_004213077.1 hypothetical protein -
M0537_RS27180 (M0537_27145) 159282..159572 + 291 WP_004213078.1 hypothetical protein -
M0537_RS27185 (M0537_27150) 159562..160461 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA 1..229626 229626


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T242933 WP_004213072.1 NZ_CP096235:c156457-156014 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T242933 NZ_OW968111:2671803-2671910 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT242933 WP_004213073.1 NZ_CP096235:c156684-156454 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT242933 NZ_OW968111:c2671750-2671689 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References