Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5386420..5387045 Replicon chromosome
Accession NZ_CP096234
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02357

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag M0537_RS25930 Protein ID WP_012737091.1
Coordinates 5386420..5386803 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M0537_RS25935 Protein ID WP_004150355.1
Coordinates 5386803..5387045 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0537_RS25915 (5383786) 5383786..5384688 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M0537_RS25920 (5384685) 5384685..5385320 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M0537_RS25925 (5385317) 5385317..5386246 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M0537_RS25930 (5386420) 5386420..5386803 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
M0537_RS25935 (5386803) 5386803..5387045 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M0537_RS25940 (5387250) 5387250..5388167 + 918 WP_012737090.1 alpha/beta hydrolase -
M0537_RS25945 (5388181) 5388181..5389122 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M0537_RS25950 (5389167) 5389167..5389604 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M0537_RS25955 (5389601) 5389601..5390461 - 861 WP_004146232.1 virulence factor BrkB family protein -
M0537_RS25960 (5390455) 5390455..5391054 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T242930 WP_012737091.1 NZ_CP096234:c5386803-5386420 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T242930 NZ_OW968111:2008479-2008582 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT242930 WP_004150355.1 NZ_CP096234:c5387045-5386803 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT242930 NZ_OW968111:c2008620-2008349 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References