Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4749248..4750058 Replicon chromosome
Accession NZ_CP096234
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02357

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag M0537_RS22955 Protein ID WP_014908042.1
Coordinates 4749248..4749781 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M0537_RS22960 Protein ID WP_002887278.1
Coordinates 4749792..4750058 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0537_RS22950 (4748079) 4748079..4749200 + 1122 WP_012737335.1 cupin domain-containing protein -
M0537_RS22955 (4749248) 4749248..4749781 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
M0537_RS22960 (4749792) 4749792..4750058 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M0537_RS22965 (4750161) 4750161..4751594 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
M0537_RS22970 (4751584) 4751584..4752267 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
M0537_RS22975 (4752439) 4752439..4753824 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
M0537_RS22980 (4753842) 4753842..4754186 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T242928 WP_014908042.1 NZ_CP096234:c4749781-4749248 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T242928 NZ_OW968111:1835559-1835810 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT242928 WP_002887278.1 NZ_CP096234:c4750058-4749792 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT242928 NZ_OW968111:1835807-1836061 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References