Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4157022..4157641 Replicon chromosome
Accession NZ_CP096234
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02357

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag M0537_RS20110 Protein ID WP_002892050.1
Coordinates 4157423..4157641 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag M0537_RS20105 Protein ID WP_002892066.1
Coordinates 4157022..4157396 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0537_RS20095 (4152174) 4152174..4153367 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M0537_RS20100 (4153390) 4153390..4156536 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
M0537_RS20105 (4157022) 4157022..4157396 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
M0537_RS20110 (4157423) 4157423..4157641 + 219 WP_002892050.1 HHA domain-containing protein Toxin
M0537_RS20115 (4157800) 4157800..4158366 + 567 WP_002892030.1 maltose O-acetyltransferase -
M0537_RS20120 (4158338) 4158338..4158478 - 141 WP_004147370.1 hypothetical protein -
M0537_RS20125 (4158499) 4158499..4158969 + 471 WP_002892026.1 YlaC family protein -
M0537_RS20130 (4158944) 4158944..4160395 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
M0537_RS20135 (4160496) 4160496..4161194 + 699 WP_002892021.1 GNAT family protein -
M0537_RS20140 (4161191) 4161191..4161331 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
M0537_RS20145 (4161331) 4161331..4161594 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4161654..4162700 1046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T242927 WP_002892050.1 NZ_CP096234:4157423-4157641 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T242927 NZ_OW968111:1328447-1328845 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT242927 WP_002892066.1 NZ_CP096234:4157022-4157396 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT242927 NZ_OW968111:1328220-1328447 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References