Detailed information of TA system TA024291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3039344..3039969
• Replicon: chromosome
• Accession: NC_013353
• Organism: Escherichia coli O103:H2 str. 12009

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ECO103_RS15765
• Protein ID: WP_000911330.1
• Coordinates: 3039344..3039742 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: ECO103_RS15770
• Protein ID: WP_000450524.1
• Coordinates: 3039742..3039969 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO103_RS15745	3035222..3035422	+	201	WP_000383836.1	YpfN family protein	-
ECO103_RS15750	3035532..3036230	-	699	WP_000679812.1	esterase	-
ECO103_RS15755	3036304..3038319	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
ECO103_RS15760	3038334..3039197	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
ECO103_RS15765	3039344..3039742	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ECO103_RS15770	3039742..3039969	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
ECO103_RS15775	3040123..3040836	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
ECO103_RS15785	3041049..3042083	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
ECO103_RS15790	3042100..3042978	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
ECO103_RS15795	3043124..3043696	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
ECO103_RS15800	3043696..3044166	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T24291 WP_000911330.1 NC_013353:c3039742-3039344 [Escherichia coli O103:H2 str. 12009]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T24291 NC_013353:c3039742-3039344 [Escherichia coli O103:H2 str. 12009]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT24291 WP_000450524.1 NC_013353:c3039969-3039742 [Escherichia coli O103:H2 str. 12009]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT24291 NC_013353:c3039969-3039742 [Escherichia coli O103:H2 str. 12009]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33