Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5373188..5373813 Replicon chromosome
Accession NZ_CP096230
Organism Klebsiella pneumoniae subsp. pneumoniae strain H39

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag M0538_RS25910 Protein ID WP_012737091.1
Coordinates 5373188..5373571 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M0538_RS25915 Protein ID WP_004150355.1
Coordinates 5373571..5373813 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0538_RS25895 (5370554) 5370554..5371456 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M0538_RS25900 (5371453) 5371453..5372088 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M0538_RS25905 (5372085) 5372085..5373014 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M0538_RS25910 (5373188) 5373188..5373571 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
M0538_RS25915 (5373571) 5373571..5373813 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M0538_RS25920 (5374018) 5374018..5374935 + 918 WP_012737090.1 alpha/beta hydrolase -
M0538_RS25925 (5374949) 5374949..5375890 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M0538_RS25930 (5375935) 5375935..5376372 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M0538_RS25935 (5376369) 5376369..5377229 - 861 WP_004146232.1 virulence factor BrkB family protein -
M0538_RS25940 (5377223) 5377223..5377822 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T242909 WP_012737091.1 NZ_CP096230:c5373571-5373188 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T242909 NZ_OW968011:c1937281-1936946 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT242909 WP_004150355.1 NZ_CP096230:c5373813-5373571 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT242909 NZ_OW968011:c1937529-1937281 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References