Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4150099..4150718 Replicon chromosome
Accession NZ_CP096230
Organism Klebsiella pneumoniae subsp. pneumoniae strain H39

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag M0538_RS20095 Protein ID WP_002892050.1
Coordinates 4150500..4150718 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag M0538_RS20090 Protein ID WP_002892066.1
Coordinates 4150099..4150473 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0538_RS20080 (4145251) 4145251..4146444 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M0538_RS20085 (4146467) 4146467..4149613 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
M0538_RS20090 (4150099) 4150099..4150473 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
M0538_RS20095 (4150500) 4150500..4150718 + 219 WP_002892050.1 HHA domain-containing protein Toxin
M0538_RS20100 (4150877) 4150877..4151443 + 567 WP_002892030.1 maltose O-acetyltransferase -
M0538_RS20105 (4151415) 4151415..4151555 - 141 WP_004147370.1 hypothetical protein -
M0538_RS20110 (4151576) 4151576..4152046 + 471 WP_002892026.1 YlaC family protein -
M0538_RS20115 (4152021) 4152021..4153472 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
M0538_RS20120 (4153573) 4153573..4154271 + 699 WP_002892021.1 GNAT family protein -
M0538_RS20125 (4154268) 4154268..4154408 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
M0538_RS20130 (4154408) 4154408..4154671 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T242906 WP_002892050.1 NZ_CP096230:4150500-4150718 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T242906 NZ_OW968011:c1144100-1143846 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT242906 WP_002892066.1 NZ_CP096230:4150099-4150473 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT242906 NZ_OW968011:c1144348-1144097 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References