Detailed information of TA system TA242893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 51627..52285
• Replicon: plasmid pXAC64
• Accession: NZ_CP096229
• Organism: Xanthomonas citri pv. citri strain CQ13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: XCQ_RS22495
• Protein ID: WP_011053002.1
• Coordinates: 51627..52028 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M4U2L0
• Locus tag: XCQ_RS22500
• Protein ID: WP_011053003.1
• Coordinates: 52025..52285 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XCQ_RS22475 (XCQ_22475)	47067..47834	-	768	WP_011052998.1	ParA family protein	-
XCQ_RS22480 (XCQ_22480)	48639..49865	-	1227	WP_015472949.1	replication protein RepA	-
XCQ_RS22485 (XCQ_22485)	50145..51332	-	1188	WP_011053001.1	zeta toxin family protein	-
XCQ_RS22490 (XCQ_22490)	51339..51602	-	264	WP_005917846.1	hypothetical protein	-
XCQ_RS22495 (XCQ_22495)	51627..52028	-	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
XCQ_RS22500 (XCQ_22500)	52025..52285	-	261	WP_011053003.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
XCQ_RS22505 (XCQ_22505)	52476..53048	+	573	WP_011053004.1	recombinase family protein	-
XCQ_RS22510 (XCQ_22510)	53079..53689	-	611	Protein_60	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64942	64942
	-	flank	IS/Tn	-	-	52332..53048	716

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T242893 WP_011053002.1 NZ_CP096229:c52028-51627 [Xanthomonas citri pv. citri]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T242893 NZ_OW967975:c4404017-4403844 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9799.00 Da
• Isoelectric Point: 5.0941

>AT242893 WP_011053003.1 NZ_CP096229:c52285-52025 [Xanthomonas citri pv. citri]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIVLKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT242893 NZ_OW967975:c4404341-4404045 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: M4U2L0