Detailed information of TA system TA242892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 31393..31886
• Replicon: plasmid pXAC64
• Accession: NZ_CP096229
• Organism: Xanthomonas citri pv. citri strain CQ13

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: XCQ_RS22365
• Protein ID: WP_015462614.1
• Coordinates: 31393..31719 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: XCQ_RS22370
• Protein ID: WP_017157755.1
• Coordinates: 31716..31886 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XCQ_RS22355 (XCQ_22355)	26776..28350	+	1575	WP_011052977.1	type IV secretion system DNA-binding domain-containing protein	-
XCQ_RS22360 (XCQ_22360)	28364..31339	+	2976	WP_011052978.1	MobF family relaxase	-
XCQ_RS22365 (XCQ_22365)	31393..31719	-	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XCQ_RS22370 (XCQ_22370)	31716..31886	-	171	WP_017157755.1	antitoxin MazE family protein	Antitoxin
XCQ_RS22375 (XCQ_22375)	32071..32457	-	387	WP_003491149.1	hypothetical protein	-
XCQ_RS22380 (XCQ_22380)	32534..32953	-	420	WP_011052981.1	hypothetical protein	-
XCQ_RS22385 (XCQ_22385)	33079..33957	-	879	WP_011052982.1	lytic transglycosylase domain-containing protein	-
XCQ_RS22390 (XCQ_22390)	33968..34990	-	1023	WP_011052983.1	P-type DNA transfer ATPase VirB11	-
XCQ_RS22395 (XCQ_22395)	35001..36221	-	1221	WP_011052984.1	TrbI/VirB10 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64942	64942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (2-54)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T242892 WP_015462614.1 NZ_CP096229:c31719-31393 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T242892 NZ_OW967975:c4206790-4206413 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6518.34 Da
• Isoelectric Point: 4.1476

>AT242892 WP_017157755.1 NZ_CP096229:c31886-31716 [Xanthomonas citri pv. citri]
MAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 171 bp

>AT242892 NZ_OW967975:c4207248-4206880 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA