Detailed information of TA system TA242891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 7240..7829
• Replicon: plasmid pXAC33
• Accession: NZ_CP096228
• Organism: Xanthomonas citri pv. citri strain CQ13

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: XCQ_RS22030
• Protein ID: WP_015463824.1
• Coordinates: 7240..7572 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: XCQ_RS22035
• Protein ID: WP_011052949.1
• Coordinates: 7572..7829 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XCQ_RS22010 (XCQ_22010)	5720..5941	-	222	WP_011052944.1	hypothetical protein	-
XCQ_RS22015 (XCQ_22015)	6231..6767	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
XCQ_RS22020 (XCQ_22020)	6757..6969	-	213	WP_011052947.1	hypothetical protein	-
XCQ_RS22025 (XCQ_22025)	6966..7217	-	252	WP_125459571.1	hypothetical protein	-
XCQ_RS22030 (XCQ_22030)	7240..7572	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XCQ_RS22035 (XCQ_22035)	7572..7829	-	258	WP_011052949.1	antitoxin	Antitoxin
XCQ_RS22040 (XCQ_22040)	8035..8637	+	603	WP_011052950.1	recombinase family protein	-
XCQ_RS22045 (XCQ_22045)	8621..8925	+	305	Protein_9	Tn3 family transposase	-
XCQ_RS22050 (XCQ_22050)	9092..9298	-	207	Protein_10	Tn3 family transposase	-
XCQ_RS22055 (XCQ_22055)	9343..9912	-	570	Protein_11	UPF0149 family protein	-
XCQ_RS22060 (XCQ_22060)	9931..11145	-	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
XCQ_RS22065 (XCQ_22065)	11219..11401	-	183	Protein_13	UPF0149 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34102	34102

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T242891 WP_015463824.1 NZ_CP096228:c7572-7240 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T242891 NZ_OW967975:c4136174-4135887 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT242891 WP_011052949.1 NZ_CP096228:c7829-7572 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT242891 NZ_OW967975:c4135900-4135598 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6