Detailed information of TA system TA024289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2327226..2327704
• Replicon: chromosome
• Accession: NC_013353
• Organism: Escherichia coli O103:H2 str. 12009

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: ECO103_RS12000
• Protein ID: WP_001303876.1
• Coordinates: 2327417..2327704 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: ECO103_RS11995
• Protein ID: WP_000536233.1
• Coordinates: 2327226..2327417 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO103_RS11960	2322606..2323376	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ECO103_RS11965	2323458..2324144	-	687	WP_000788749.1	ATP-binding protein	-
ECO103_RS11970	2324151..2325266	-	1116	WP_001205820.1	hypothetical protein	-
ECO103_RS29750	2325345..2325800	+	456	Protein_2283	hypothetical protein	-
ECO103_RS11980	2326007..2326432	-	426	WP_000693855.1	Rha family transcriptional regulator	-
ECO103_RS11985	2326416..2326688	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
ECO103_RS11990	2326797..2327198	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
ECO103_RS11995	2327226..2327417	+	192	WP_000536233.1	hypothetical protein	Antitoxin
ECO103_RS12000	2327417..2327704	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECO103_RS12005	2327981..2328136	+	156	WP_000379575.1	DUF1391 family protein	-
ECO103_RS12010	2328278..2328667	+	390	WP_000394511.1	hypothetical protein	-
ECO103_RS12015	2328854..2329039	-	186	WP_001133046.1	hypothetical protein	-
ECO103_RS12025	2329613..2329801	+	189	WP_000413705.1	cell division inhibition protein DicB	-
ECO103_RS12030	2329798..2329989	+	192	WP_001098307.1	DUF1482 family protein	-
ECO103_RS12035	2330083..2332554	+	2472	WP_000034468.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleH2 / nleC / nleB2 / espK / espFu/tccP	2284149..2439873	155724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T24289 WP_001303876.1 NC_013353:2327417-2327704 [Escherichia coli O103:H2 str. 12009]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T24289 NC_013353:2327417-2327704 [Escherichia coli O103:H2 str. 12009]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT24289 WP_000536233.1 NC_013353:2327226-2327417 [Escherichia coli O103:H2 str. 12009]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT24289 NC_013353:2327226-2327417 [Escherichia coli O103:H2 str. 12009]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA