Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 39911..40518 Replicon plasmid pUCH5-2
Accession NZ_CP096221
Organism Enterococcus faecium strain UCH5

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag M0F39_RS14690 Protein ID WP_002321032.1
Coordinates 39911..40261 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag M0F39_RS14695 Protein ID WP_002287514.1
Coordinates 40255..40518 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0F39_RS14655 (M0F39_14655) 35204..35611 - 408 WP_002305774.1 hypothetical protein -
M0F39_RS14660 (M0F39_14660) 35630..36448 - 819 WP_002305212.1 ParA family protein -
M0F39_RS14665 (M0F39_14665) 36674..36967 - 294 WP_002322251.1 hypothetical protein -
M0F39_RS14670 (M0F39_14670) 36979..37158 - 180 WP_002322386.1 hypothetical protein -
M0F39_RS14675 (M0F39_14675) 37155..37505 - 351 WP_000568378.1 hypothetical protein -
M0F39_RS14680 (M0F39_14680) 37498..38823 - 1326 WP_129992446.1 Y-family DNA polymerase -
M0F39_RS14685 (M0F39_14685) 39273..39887 + 615 WP_002318230.1 recombinase family protein -
M0F39_RS14690 (M0F39_14690) 39911..40261 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M0F39_RS14695 (M0F39_14695) 40255..40518 - 264 WP_002287514.1 hypothetical protein Antitoxin
M0F39_RS14700 (M0F39_14700) 40756..41901 - 1146 WP_002305757.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
M0F39_RS14705 (M0F39_14705) 41913..42314 - 402 WP_002305759.1 IS200/IS605 family transposase -
M0F39_RS14710 (M0F39_14710) 42487..42753 - 267 Protein_48 hypothetical protein -
M0F39_RS14715 (M0F39_14715) 42735..43151 - 417 WP_002305764.1 hypothetical protein -
M0F39_RS14720 (M0F39_14720) 43167..43418 - 252 WP_002305766.1 hypothetical protein -
M0F39_RS14725 (M0F39_14725) 43418..43624 - 207 WP_002296239.1 hypothetical protein -
M0F39_RS14730 (M0F39_14730) 44069..44329 - 261 WP_002321303.1 hypothetical protein -
M0F39_RS14735 (M0F39_14735) 44695..45168 - 474 WP_021414961.1 single-stranded DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..54822 54822


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T242885 WP_002321032.1 NZ_CP096221:c40261-39911 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T242885 NZ_OW967975:c2500367-2500191 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT242885 WP_002287514.1 NZ_CP096221:c40518-40255 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT242885 NZ_OW967975:c2500145-2499729 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References