Detailed information of TA system TA242883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 489399..489970
• Replicon: chromosome
• Accession: NZ_CP096219
• Organism: Enterococcus faecium strain UCH5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3Y2B8
• Locus tag: M0F39_RS02310
• Protein ID: WP_002286801.1
• Coordinates: 489629..489970 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: M0F39_RS02305
• Protein ID: WP_033659738.1
• Coordinates: 489399..489629 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0F39_RS02280 (M0F39_02280)	484532..485863	+	1332	WP_002286818.1	FAD-containing oxidoreductase	-
M0F39_RS02285 (M0F39_02285)	485885..486511	+	627	WP_002286816.1	cysteine hydrolase	-
M0F39_RS02290 (M0F39_02290)	486694..487275	+	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
M0F39_RS02295 (M0F39_02295)	488007..488569	+	563	Protein_456	SOS response-associated peptidase family protein	-
M0F39_RS02300 (M0F39_02300)	488774..489112	-	339	WP_002286804.1	hypothetical protein	-
M0F39_RS02305 (M0F39_02305)	489399..489629	+	231	WP_033659738.1	hypothetical protein	Antitoxin
M0F39_RS02310 (M0F39_02310)	489629..489970	+	342	WP_002286801.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M0F39_RS02315 (M0F39_02315)	491003..492190	-	1188	WP_002296840.1	IS256-like element IS16 family transposase	-
M0F39_RS02320 (M0F39_02320)	492984..493178	+	195	WP_002297028.1	hypothetical protein	-
M0F39_RS02325 (M0F39_02325)	493369..493618	+	250	Protein_462	transposase	-
M0F39_RS02330 (M0F39_02330)	493862..494692	-	831	WP_002286789.1	manganese catalase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	491003..492190	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13302.68 Da
• Isoelectric Point: 9.9044

>T242883 WP_002286801.1 NZ_CP096219:489629-489970 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T242883 NZ_OW967975:2294830-2295117 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8451.32 Da
• Isoelectric Point: 3.9490

>AT242883 WP_033659738.1 NZ_CP096219:489399-489629 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIGDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT242883 NZ_OW967975:2294591-2294830 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A829FD66