Detailed information of TA system TA242881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 33325..33844
• Replicon: plasmid pUCH1-2
• Accession: NZ_CP096213
• Organism: Enterococcus faecium strain UCH1

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: M0E25_RS16160
• Protein ID: WP_000588503.1
• Coordinates: 33587..33844 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: M0E25_RS16155
• Protein ID: WP_000388479.1
• Coordinates: 33325..33594 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0E25_RS16115 (M0E25_16115)	28473..29180	+	708	Protein_33	replication initiation protein	-
M0E25_RS16120 (M0E25_16120)	29317..29733	-	417	WP_001232859.1	hypothetical protein	-
M0E25_RS16125 (M0E25_16125)	29736..30020	-	285	WP_000353844.1	hypothetical protein	-
M0E25_RS16130 (M0E25_16130)	30388..30612	+	225	WP_000410576.1	hypothetical protein	-
M0E25_RS16135 (M0E25_16135)	31714..32394	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
M0E25_RS16140 (M0E25_16140)	32470..32634	+	165	Protein_38	hypothetical protein	-
M0E25_RS16145 (M0E25_16145)	32799..32999	+	201	WP_001033544.1	4-hydroxybenzoate octaprenyltransferase	-
M0E25_RS16150 (M0E25_16150)	33015..33194	+	180	WP_001120991.1	hypothetical protein	-
M0E25_RS16155 (M0E25_16155)	33325..33594	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
M0E25_RS16160 (M0E25_16160)	33587..33844	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
M0E25_RS16165 (M0E25_16165)	34482..35306	+	825	WP_001809248.1	hypothetical protein	-
M0E25_RS16170 (M0E25_16170)	35471..36085	-	615	WP_001261742.1	recombinase family protein	-
M0E25_RS16175 (M0E25_16175)	36323..36745	+	423	WP_002303116.1	hypothetical protein	-
M0E25_RS16180 (M0E25_16180)	36755..36958	+	204	WP_002299573.1	hypothetical protein	-
M0E25_RS16185 (M0E25_16185)	37169..37753	+	585	WP_094850611.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / VanHAX	-	1..42009	42009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T242881 WP_000588503.1 NZ_CP096213:33587-33844 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T242881 NZ_OW967975:1796502-1796756 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT242881 WP_000388479.1 NZ_CP096213:33325-33594 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT242881 NZ_OW967975:1796254-1796505 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7