Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 181350..181957 Replicon plasmid pUCH1-1
Accession NZ_CP096212
Organism Enterococcus faecium strain UCH1

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag M0E25_RS15330 Protein ID WP_002321032.1
Coordinates 181350..181700 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag M0E25_RS15335 Protein ID WP_002287514.1
Coordinates 181694..181957 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0E25_RS15295 (M0E25_15295) 176617..177351 + 735 WP_010729798.1 GntR family transcriptional regulator -
M0E25_RS15300 (M0E25_15300) 177335..178045 + 711 WP_010729797.1 GntR family transcriptional regulator -
M0E25_RS15305 (M0E25_15305) 178099..178779 + 681 WP_199023549.1 IS6-like element IS1216 family transposase -
M0E25_RS15310 (M0E25_15310) 178968..179552 - 585 WP_094850611.1 recombinase family protein -
M0E25_RS15315 (M0E25_15315) 179763..179966 - 204 WP_002299573.1 hypothetical protein -
M0E25_RS15320 (M0E25_15320) 179976..180398 - 423 WP_002303116.1 hypothetical protein -
M0E25_RS15325 (M0E25_15325) 180712..181326 + 615 WP_002318230.1 recombinase family protein -
M0E25_RS15330 (M0E25_15330) 181350..181700 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M0E25_RS15335 (M0E25_15335) 181694..181957 - 264 WP_002287514.1 hypothetical protein Antitoxin
M0E25_RS15340 (M0E25_15340) 182195..183340 - 1146 WP_002305757.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
M0E25_RS15345 (M0E25_15345) 183352..183753 - 402 WP_002305759.1 IS200/IS605 family transposase -
M0E25_RS15350 (M0E25_15350) 184163..184342 - 180 WP_002305761.1 hypothetical protein -
M0E25_RS15355 (M0E25_15355) 184723..185358 - 636 WP_002305763.1 hypothetical protein -
M0E25_RS15360 (M0E25_15360) 185340..185756 - 417 WP_002305764.1 hypothetical protein -
M0E25_RS15365 (M0E25_15365) 185772..186023 - 252 WP_002305766.1 hypothetical protein -
M0E25_RS15370 (M0E25_15370) 186023..186229 - 207 WP_002296239.1 hypothetical protein -
M0E25_RS15375 (M0E25_15375) 186674..186934 - 261 WP_002321303.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..291523 291523
- inside IScluster/Tn - - 178099..183753 5654


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T242879 WP_002321032.1 NZ_CP096212:c181700-181350 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T242879 NZ_OW967975:999950-1000285 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT242879 WP_002287514.1 NZ_CP096212:c181957-181694 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT242879 NZ_OW967975:999702-999950 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References