Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2285715..2286286 Replicon chromosome
Accession NZ_CP096211
Organism Enterococcus faecium strain UCH1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag M0E25_RS11105 Protein ID WP_002286801.1
Coordinates 2285715..2286056 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag M0E25_RS11110 Protein ID WP_002323011.1
Coordinates 2286056..2286286 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0E25_RS11080 (M0E25_11080) 2280960..2281790 + 831 WP_002286789.1 manganese catalase family protein -
M0E25_RS11085 (M0E25_11085) 2282034..2282283 - 250 Protein_2183 transposase -
M0E25_RS11090 (M0E25_11090) 2282474..2282668 - 195 WP_002297028.1 hypothetical protein -
M0E25_RS11095 (M0E25_11095) 2283173..2283358 - 186 WP_002304207.1 hypothetical protein -
M0E25_RS11100 (M0E25_11100) 2284187..2285479 + 1293 WP_000122610.1 ISL3-like element IS1251 family transposase -
M0E25_RS11105 (M0E25_11105) 2285715..2286056 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M0E25_RS11110 (M0E25_11110) 2286056..2286286 - 231 WP_002323011.1 hypothetical protein Antitoxin
M0E25_RS11115 (M0E25_11115) 2286573..2286911 + 339 WP_002286804.1 hypothetical protein -
M0E25_RS11120 (M0E25_11120) 2287116..2287678 - 563 Protein_2190 SOS response-associated peptidase family protein -
M0E25_RS11125 (M0E25_11125) 2288410..2288991 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
M0E25_RS11130 (M0E25_11130) 2289174..2289800 - 627 WP_002286816.1 cysteine hydrolase -
M0E25_RS11135 (M0E25_11135) 2289822..2291153 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2284187..2285479 1292


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T242878 WP_002286801.1 NZ_CP096211:c2286056-2285715 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T242878 NZ_OW967975:859591-859998 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT242878 WP_002323011.1 NZ_CP096211:c2286286-2286056 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT242878 NZ_OW967975:859344-859610 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References