Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1205357..1206032 Replicon chromosome
Accession NZ_CP096211
Organism Enterococcus faecium strain UCH1

Toxin (Protein)


Gene name hicA Uniprot ID A0A430A2J6
Locus tag M0E25_RS05805 Protein ID WP_002350171.1
Coordinates 1205357..1205539 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A430A2G3
Locus tag M0E25_RS05810 Protein ID WP_042958047.1
Coordinates 1205577..1206032 (+) Length 152 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0E25_RS05775 (M0E25_05775) 1200993..1201607 + 615 WP_002350165.1 hypothetical protein -
M0E25_RS05780 (M0E25_05780) 1201748..1202038 + 291 WP_042958053.1 thioredoxin family protein -
M0E25_RS05785 (M0E25_05785) 1202060..1202875 + 816 WP_002350167.1 hypothetical protein -
M0E25_RS05790 (M0E25_05790) 1203238..1203495 + 258 WP_002350168.1 ribbon-helix-helix protein, CopG family -
M0E25_RS05795 (M0E25_05795) 1203495..1204043 + 549 WP_002350169.1 hypothetical protein -
M0E25_RS05800 (M0E25_05800) 1204067..1205287 + 1221 WP_230079092.1 MobP2 family relaxase -
M0E25_RS05805 (M0E25_05805) 1205357..1205539 + 183 WP_002350171.1 type II toxin-antitoxin system HicA family toxin Toxin
M0E25_RS05810 (M0E25_05810) 1205577..1206032 + 456 WP_042958047.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
M0E25_RS05815 (M0E25_05815) 1206127..1206567 + 441 WP_002350173.1 hypothetical protein -
M0E25_RS05820 (M0E25_05820) 1206639..1208222 + 1584 WP_002350174.1 DUF3991 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(7-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(18-116)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6498.85 Da        Isoelectric Point: 11.3109

>T242877 WP_002350171.1 NZ_CP096211:1205357-1205539 [Enterococcus faecium]
MPMTQNEMVKLLIEHGGVKVKGGKGSHIKVRFPGVNRPIIVPQKLPKGTENGILKQAGLK

Download         Length: 183 bp

>T242877 NZ_OW967975:714558-714854 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 152 a.a.        Molecular weight: 16865.84 Da        Isoelectric Point: 4.0033

>AT242877 WP_042958047.1 NZ_CP096211:1205577-1206032 [Enterococcus faecium]
MLVSYPALFYFENSEGYDPGFSVFFPDFPNTGGTSGSTISEALEMASDYLGILLSDDIEQERELPKPSLISSLSLEENDP
FKDDSDFNFEYDFEKSFVSMVSVDLSQYLGNNDPIKKTLTIPKWADKLGKDLHLNFSKTLTDAIVSKKMGA

Download         Length: 456 bp

>AT242877 NZ_OW967975:714856-715251 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A430A2J6

Antitoxin

Source ID Structure
AlphaFold DB A0A430A2G3

References